Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-deoxy-D-arabino-heptonic acid 7-phosphate | - |
Escherichia coli | |
D-fructose 1,6-diphosphate | - |
Escherichia coli | |
D-sedoheptulose 1,7-diphosphate | - |
Escherichia coli | |
D-sedoheptulose 7-phosphate | - |
Escherichia coli | |
p-chloromercuribenzoate | complete inhibition at 0.02 mM, reversible by cysteine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
D-erythrose 4-phosphate | pH 6.4, 37°C | Escherichia coli | |
3.5 | - |
phosphoenolpyruvate | pH 6.4, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
mutant 83-24 | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
partially purified enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-15°C, ammonium sulfate and acetone fractions, stable for at least several weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with D-erythrose, D-glyceraldehyde 3-phosphate, ribose 5-phosphate, glucose 6-phosphate, glucosamine 6-phosphate, N-acetylglucosamine 6-phosphate, and pyruvate | Escherichia coli | ? | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Escherichia coli | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Escherichia coli |