Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.47 extracted from

  • Uda, N.; Matoba, Y.; Oda, K.; Kumagai, T.; Sugiyama, M.
    The structural and mutational analyses of O-ureido-L-serine synthase necessary for D-cycloserine biosynthesis (2015), FEBS J., 282, 3929-3944.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces lavendulae

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type and mutant K43A, to 2.25 and 1.9 A resolution, respectively. Each monomer of DcsD takes a typical fold of type II pyridoxal 5'-phosphate enzymes with the cofactor pyridoxal 5'-phosphate covalently bound to invariant Lys residue (Lys43) at the active site. The pyridine ring of pyridoxal 5'-phoshate makes hydrogen bonds with invariant Asn73 and Ser265 residues. Its phosphate group makes hydrogen bonds with Gly177, Thr178, Thr179 and Thr181 residues Streptomyces lavendulae

Protein Variants

Protein Variants Comment Organism
K43A mutation in invariant Lys43 residue, inactive. Mutant forms an external aldimine adduct upon addition of L-methionine or O-ureido-L-serine Streptomyces lavendulae
S121A decreased activity for synthesis of ureido-L-serine, increased activity for synthesis of L-cysteine Streptomyces lavendulae
S121M decreased activity for synthesis of ureido-L-serine, increased activity for synthesis of L-cysteine Streptomyces lavendulae
V74T decreased activity for synthesis of ureido-L-serine, increased activity for synthesis of L-cysteine Streptomyces lavendulae
Y97F decreased activity for synthesis of ureido-L-serine, increased activity for synthesis of L-cysteine Streptomyces lavendulae
Y97M decreased activity for synthesis of ureido-L-serine, increased activity for synthesis of L-cysteine Streptomyces lavendulae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
H2S mutant Y97F, pH 8.0, 30°C Streptomyces lavendulae
0.084
-
H2S mutant Y97M, pH 8.0, 30°C Streptomyces lavendulae
0.095
-
H2S mutant S121A, pH 8.0, 30°C Streptomyces lavendulae
0.12
-
H2S wild-type, pH 8.0, 30°C Streptomyces lavendulae
0.14
-
H2S mutant V74T, pH 8.0, 30°C Streptomyces lavendulae
0.2
-
H2S mutant S121M, pH 8.0, 30°C Streptomyces lavendulae

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae D2Z027
-
-
Streptomyces lavendulae ATCC 11924 D2Z027
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme additionally catalyzes the formation of O-ureido-L-serine from O-acetyl-L-serine and hydroxyurea, reaction of EC 2.6.99.3. The kcat/Km value of DcsD for L-cysteine synthesis is 80fold higher than that for O-ureido-L-serine synthesis Streptomyces lavendulae ?
-
?
additional information enzyme additionally catalyzes the formation of O-ureido-L-serine from O-acetyl-L-serine and hydroxyurea, reaction of EC 2.6.99.3. The kcat/Km value of DcsD for L-cysteine synthesis is 80fold higher than that for O-ureido-L-serine synthesis Streptomyces lavendulae ATCC 11924 ?
-
?
O-acetyl-L-serine
-
Streptomyces lavendulae L-cysteine + acetate
-
?
O-acetyl-L-serine
-
Streptomyces lavendulae ATCC 11924 L-cysteine + acetate
-
?

Subunits

Subunits Comment Organism
tetramer both in solution and in a crystalline state, crystallization data Streptomyces lavendulae

Synonyms

Synonyms Comment Organism
DcsD
-
Streptomyces lavendulae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
H2S mutant Y97F, pH 8.0, 30°C Streptomyces lavendulae
12
-
H2S wild-type, pH 8.0, 30°C Streptomyces lavendulae
15
-
H2S mutant S121A, pH 8.0, 30°C Streptomyces lavendulae
22
-
H2S mutant Y97M, pH 8.0, 30°C Streptomyces lavendulae
26
-
H2S mutant V74T, pH 8.0, 30°C Streptomyces lavendulae
44
-
H2S mutant S121M, pH 8.0, 30°C Streptomyces lavendulae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Streptomyces lavendulae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
100
-
H2S wild-type, pH 8.0, 30°C Streptomyces lavendulae
140
-
H2S mutant Y97F, pH 8.0, 30°C Streptomyces lavendulae
160
-
H2S mutant S121A, pH 8.0, 30°C Streptomyces lavendulae
190
-
H2S mutant V74T, pH 8.0, 30°C Streptomyces lavendulae
220
-
H2S mutant S121M, pH 8.0, 30°C Streptomyces lavendulae
260
-
H2S mutant Y97M, pH 8.0, 30°C Streptomyces lavendulae