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Literature summary for 2.5.1.47 extracted from

  • Yafuso, J.T.; Negi, V.S.; Bingham, J.P.; Borthakur, D.
    An O-acetylserine (thiol) lyase from Leucaena leucocephala is a cysteine synthase but not a mimosine synthase (2014), Appl. Biochem. Biotechnol., 173, 1157-1168.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Cy-OAS-TL, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLYsS Leucaena leucocephala

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics and modeling Leucaena leucocephala
1.46
-
hydrogen sulfide pH 7.5, 22°C, recombinant enzyme, Lineweaver-Burke model Leucaena leucocephala
1.85
-
hydrogen sulfide pH 7.5, 22°C, recombinant enzymem, Michaelis-Menten model Leucaena leucocephala

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Leucaena leucocephala 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
-
Leucaena leucocephala
40000
-
-
Leucaena leucocephala

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-acetyl-L-serine + hydrogen sulfide Leucaena leucocephala
-
L-cysteine + acetate
-
?

Organism

Organism UniProt Comment Textmining
Leucaena leucocephala W5S3I4 gene Cy-OAS-TL
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLYsS by nickel affinity chhromatography Leucaena leucocephala

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no synthesis of mimosine. The enzyme is specific for cysteine synthesis. The recombinant enzyme is active with or without the leader peptide Leucaena leucocephala ?
-
?
O-acetyl-L-serine + hydrogen sulfide
-
Leucaena leucocephala L-cysteine + acetate
-
?

Subunits

Subunits Comment Organism
? x * 40000, recombinant His-tagged enzyme, SDS-PAGE, x * 34000, about, sequence calculation of untagged enzyme Leucaena leucocephala

Synonyms

Synonyms Comment Organism
O-acetylserine (thiol) lyase
-
Leucaena leucocephala
OAS-TL
-
Leucaena leucocephala

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Leucaena leucocephala

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Leucaena leucocephala

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Leucaena leucocephala

General Information

General Information Comment Organism
physiological function tree legume Leucaena contains a toxic, nonprotein amino acid, mimosine, which is not formed by the enzyme O-acetylserine (thiol) lyase, OAS-TL Leucaena leucocephala