Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.47 extracted from

  • Westrop, G.D.; Goodall, G.; Mottram, J.C.; Coombs, G.H.
    Cysteine biosynthesis in Trichomonas vaginalis involves cysteine synthase utilizing O-phosphoserine (2006), J. Biol. Chem., 281, 25062-25075.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.47

Application

Application Comment Organism
medicine humans lack cysteine synthase. Therefore, this parasite enzyme could be an exploitable drug target Trichomonas vaginalis

Cloned(Commentary)

Cloned (Comment) Organism
-
 Trichomonas vaginalis

Protein Variants

Protein Variants Comment Organism
K214A mutant retains high activity with O-acetylserine and sulfide (40% of the activity of the wild type enzyme), but its activity with O-phosphoserine and sulfide is reduced by more than 100fold. The ability to use thiosulfate as an alternative nucleophile in the sulfhydrylase reaction is greatly reduced, but the mutant shows no change in cysteine desulfurase activity Trichomonas vaginalis
K43A protein is yellow, indicating that it binds pyridoxal 5'-phosphate but has no detectable activity as a cysteine synthase with O-acetylserine or O-phosphoserine and no detectable cysteine desulfurase activity Trichomonas vaginalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 H2S 37°C, pH 7.8, cosubstrate: O-phosphoserine Trichomonas vaginalis
0.8
-
 L-Cys 37°C, pH 7.8 Trichomonas vaginalis
0.8
-
 H2S 37°C, pH 7.8, cosubstrate: O-acetyl-L-Ser Trichomonas vaginalis
39.5
-
 O-acetyl-L-Ser 37°C, pH 7.8 Trichomonas vaginalis
227
-
 O-phosphoserine 37°C, pH 7.8 Trichomonas vaginalis

Organism

Organism UniProt Comment Textmining
Trichomonas vaginalis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
98.5
-
 reaction with O-phosphoserine Trichomonas vaginalis
180
-
 reaction with O-acetyl-L-Ser Trichomonas vaginalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-Ser + H2S
-
 Trichomonas vaginalis L-Cys + acetate
-
 r
O-acetyl-L-serine
-
 Trichomonas vaginalis L-cysteine + acetate
-
 ?
O-phosphoserine + H2S
-
 Trichomonas vaginalis L-Cys + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
TvCS1
-
 Trichomonas vaginalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.6
-
 L-Cys 37°C, pH 7.8 Trichomonas vaginalis
153
-
 O-acetyl-L-Ser 37°C, pH 7.8 Trichomonas vaginalis
165
-
 O-phosphoserine 37°C, pH 7.8 Trichomonas vaginalis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Trichomonas vaginalis