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Literature summary for 2.5.1.47 extracted from
- O-Acetylserine sulfhydrylase from Methanosarcina thermophila (2000), J. Bacteriol., 182, 45-50.
General Stability
| General Stability | Organism |
|---|---|
| stable to several freeze-thaw cycles | Methanosarcina thermophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| O-acetylserine | substrate inhibition | Methanosarcina thermophila |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
H2S | 40°C | Methanosarcina thermophila | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * or 3 * 36000, SDS-PAGE | Methanosarcina thermophila |
| 36000 | - |
3 or 2 * 36000, SDS-PAGE | Methanosarcina thermophila |
| 93000 | - |
gel filtration | Methanosarcina thermophila |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-Ser + sulfide | Methanosarcina thermophila | final step in Cys synthesis | L-Cys + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina thermophila | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanosarcina thermophila |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.129 | - |
- |
Methanosarcina thermophila |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20 C, complete loss of activity after 2 months | Methanosarcina thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-Ser + H2S | - |
Methanosarcina thermophila | L-Cys + acetate | - |
? | |
| O-acetyl-L-Ser + sulfide | final step in Cys synthesis | Methanosarcina thermophila | L-Cys + acetate | - |
? | |
| O-acetyl-L-serine | - |
Methanosarcina thermophila | L-cysteine + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * or 3 * 36000, SDS-PAGE | Methanosarcina thermophila |
| trimer | 3 or 2 * 36000, SDS-PAGE | Methanosarcina thermophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | - |
Methanosarcina thermophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | - |
pH 8.0, 50 mM dithiothreitol, 0.08 mM pyridoxal phosphate, 30 min, stable | Methanosarcina thermophila |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | enzyme contains pyridoxal phosphate | Methanosarcina thermophila | |
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Release 2023.1 (January 2023)
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