Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.47 extracted from

  • Yamagata, S.; Takeshima, K.
    O-Acetylserine and O-acetylhomoserine sulfhydrylase of yeast. Further purification and characterization as a pyridoxal enzyme (1976), J. Biochem., 80, 777-785.
    View publication on PubMed
Search for more literature for 2.5.1.47

Inhibitors

Inhibitors Comment Organism Structure
pyridoxal
-
 Saccharomyces cerevisiae
pyridoxal hydrochloride 54% inhibition at 1 mM Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-acetyl-L-Ser + sulfide Saccharomyces cerevisiae functions as a Cys synthase rather than as a homocysteine synthase in vivo L-Cys + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate mechanism similar to those of other pyridoxal enzymes Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.2
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20 C, potassium phosphate buffer, pH 6.5, 1 mM EDTA, at least several days, no loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-Ser + sulfide functions as a Cys synthase rather than as a homocysteine synthase in vivo Saccharomyces cerevisiae L-Cys + acetate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 pyridoxal 5'-phosphate stabilizes against heat inactivation Saccharomyces cerevisiae
55
-
 5 min, 45% loss of activity, without pyridoxal 5'-phosphate Saccharomyces cerevisiae
65
-
 5 min, complete loss of activity, without pyridoxal 5'-phosphate Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate stoichiometry, binds to Lys, protects against inactivation by heat, urea, and trypsin, four binding sites per mol apoenzyme, association constant Saccharomyces cerevisiae