KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
H2S | less than | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.12 | - |
acetyl-CoA | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.12 | - |
acetyl-CoA | - |
Escherichia coli | |
0.7 | - |
L-Ser | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.7 | - |
L-Ser | - |
Escherichia coli | |
5 | - |
O-acetyl-L-Ser | free enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
20 | - |
O-acetyl-L-Ser | complex-bound enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
free enzyme, sedimentation equilibrium | Salmonella enterica subsp. enterica serovar Typhimurium |
309000 | - |
cysteine synthetase complex, sedimentation equilibrium | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-Ser + sulfide | Salmonella enterica subsp. enterica serovar Typhimurium | final step in Cys synthesis | L-Cys + acetate | - |
? | |
O-acetyl-L-Ser + sulfide | Escherichia coli | final step in Cys synthesis | L-Cys + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
one enzyme free, one associated with serine transacetylase in cysteine synthase | - |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
292 | - |
complex-bound enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
1100 | - |
free enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
-20 C, Tris-HCl buffer, pH 7.6, bovine serum albumine, 8 months, 10% loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
20 C, room temperature, Tris-HCl buffer, pH 7.6, bovine serum albumine, several days, no loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Cys + acetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | O-acetyl-L-Ser + H2S | - |
r | |
L-Cys + acetate | - |
Escherichia coli | O-acetyl-L-Ser + H2S | - |
r | |
O-acetyl-L-Ser + H2S | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-Cys + acetate | - |
r | |
O-acetyl-L-Ser + H2S | - |
Escherichia coli | L-Cys + acetate | - |
r | |
O-acetyl-L-Ser + methyl mercaptan | - |
Salmonella enterica subsp. enterica serovar Typhimurium | S-methylcysteine + acetate | product identification uncertain | ir | |
O-acetyl-L-Ser + sulfide | final step in Cys synthesis | Salmonella enterica subsp. enterica serovar Typhimurium | L-Cys + acetate | - |
? | |
O-acetyl-L-Ser + sulfide | final step in Cys synthesis | Escherichia coli | L-Cys + acetate | - |
? | |
O-acetyl-L-serine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-cysteine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
62 | - |
10 min, 5% loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
67 | - |
10 min, 50% loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
70 | - |
10 min, 70% loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
733 | - |
O-acetyl-L-Ser | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
733 | - |
O-acetyl-L-Ser | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 1 per subunit of free enzyme, 4 per of cysteine synthase complex | Salmonella enterica subsp. enterica serovar Typhimurium |