Literature summary for 2.5.1.32 extracted from

Kim, I.J.; Ko, K.C.; Nam, T.S.; Kim, Y.W.; Chung, W.I.; Kim, C.S.
Expression and activity of citrus phytoene synthase and beta-carotene hydroxylase in Escherichia coli (2003), J. Microbiol., 41, 212-218.

No PubMed abstract available

Search for more literature for 2.5.1.32

Cloned(Commentary)

Cloned (Comment)	Organism
gene crtB, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme lacking the chloroplast transit peptide in Escherichia coli strain BL21(DE3) mostly in inclusion bodies	Citrus sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	recombinant in vivo reconstitution of carotinoid biosynthesis by expression of genes crtE, crtX, crtY, crtI, and crtB encoding enzymes involved in the pathway from Pantoea ananatis and citrus genes crtB and crtZ encoding CitPsy and CitChx, i.e. phytoene synthase and beta-carotene hydroxylase, in Escherichia coli strain BL21(DE3), carotinoid pigment content analysis of recombinant bacteria, overview	Citrus sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	enzyme contains a chloroplast transit peptide	Citrus sp.	9507	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	x * 28000, about, recombinant enzyme, SDS-PAGE	Citrus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranylgeranyl diphosphate + geranylgeranyl diphosphate	Citrus sp.	enzyme is involved in carotenoid biosynthesis, pathway overview	prephytoene diphosphate + diphosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Citrus sp.	-	gene crtB	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranylgeranyl diphosphate + geranylgeranyl diphosphate	-	Citrus sp.	prephytoene diphosphate + diphosphate	-	ir
geranylgeranyl diphosphate + geranylgeranyl diphosphate	enzyme is involved in carotenoid biosynthesis, pathway overview	Citrus sp.	prephytoene diphosphate + diphosphate	-	ir

Subunits

Subunits	Comment	Organism
?	x * 28000, about, recombinant enzyme, SDS-PAGE	Citrus sp.

Synonyms

Synonyms	Comment	Organism
CitPsy	-	Citrus sp.
PSY	-	Citrus sp.

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)