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Literature summary for 2.5.1.32 extracted from

  • Dogbo, O.; Laferriere, A.; D'Harlingue, A.; Camara, B.
    Carotenoid biosynthesis: isolation and characterization of a bifunctional enzyme catalyzing the synthesis of phytoene (1988), Proc. Natl. Acad. Sci. USA, 85, 7054-7058.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
prephytoene diphosphate pH 7.6, 30°C Capsicum annuum
0.003
-
geranylgeranyl diphosphate pH 7.6, 30°C Capsicum annuum

Localization

Localization Comment Organism GeneOntology No. Textmining
chromoplast stroma Capsicum annuum 9509
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ absolute requirement for Mn2+, half-maximal activity at 0.2 mM. No other divalent cation can substitute efficiently for Mn2+ Capsicum annuum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
1 * 47500, SDS-PAGE Capsicum annuum

Organism

Organism UniProt Comment Textmining
Capsicum annuum
-
a single polypeptide catalyzes the 2-step reaction from geranylgeranyl diphosphate to phytoene
-

Purification (Commentary)

Purification (Comment) Organism
-
Capsicum annuum

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Capsicum annuum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.067
-
-
Capsicum annuum

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl buffer, pH 7.6, 2 mM dithiothreitol, 0.3 M NaCl, 30% glycerol, stable for 1 year Capsicum annuum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + geranylgeranyl diphosphate the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene Capsicum annuum prephytoene diphosphate + diphosphate
-
?
prephytoene diphosphate the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene Capsicum annuum phytoene + diphosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 47500, SDS-PAGE Capsicum annuum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Capsicum annuum