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Literature summary for 2.5.1.30 extracted from

  • Zhang, Y.W.; Li, X.Y.; Koyama, T.
    Chain length determination of prenyltransferases: both heteromeric subunits of medium-chain (E)-prenyl diphosphate synthase are involved in the product chain length determination (2000), Biochemistry, 39, 12717-12722.
    View publication on PubMed
Search for more literature for 2.5.1.30

Protein Variants

Protein Variants Comment Organism
A79F mutant enzyme subunit-I(wild-type)/subunit-B(A79F). Comparable kinetic constants with those of the wild-type enzyme. Significantly different pattern of product distribution from that of the wild type. The major product is hexaprenyl diphosphate Bacillus subtilis
A79L mutant enzyme subunit-I(wild-type)/subunit-B(A79L). Comparable kinetic constants with those of the wild-type enzyme. Significantly different pattern of product distribution from that of the wild type. The major product is hexaprenyl diphosphate Bacillus subtilis
D97A mutant enzyme subunit-I(D97A)/subunit-II(wild-type), major products: geranylgeranyl diphosphate, farnesylgeranyl diphosphate, hexaprenyl diphosphate, heptaprenyl diphosphate Bacillus subtilis
D97A/A79F mutant enzyme subunit-I(97A)/subunit-II(A79F) forms exclusively geranylgeranyl diphosphate Bacillus subtilis
D97A/A79L mutant enzyme subunit-I(D97A)/subunit-II(A79L), the final product is farnesylgeranyl diphosphate, increase in the production of geranylgeranyl diphosphate Bacillus subtilis
additional information several amino acid residues in the larger subunits Bacillus subtilis heptaprenyl diphosphate synthase are selected for substitutions by site-directed mutagenesis and examined by combination with the corresponding wild type or mutated smaller subunits Bacillus subtilis
V76I mutant enzyme subunit-I(wild-type)/subunit-II(A79L), octaprenyl diphosphate is the final product with farnesyl diphosphate as allylic primer Bacillus subtilis
Y103S mutant enzyme subunit-I(Y103S)/subunit-II(wild-type), final product is octaprenyl diphosphate Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, wild-type enzyme Bacillus subtilis
0.0085
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-B(A79F) Bacillus subtilis
0.0116
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-B(I76G) Bacillus subtilis
0.0124
-
 (2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79L) Bacillus subtilis
0.0143
-
 isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79F) Bacillus subtilis
0.0168
-
 geranylgeranyl diphosphate pH 8.5, 37°C, wild-type enzyme Bacillus subtilis
0.0198
-
 isopentenyl diphosphate pH 8.5, 37°C, wild-type enzyme Bacillus subtilis
0.0198
-
 geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(I76G) Bacillus subtilis
0.0214
-
 isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(I76G) Bacillus subtilis
0.0259
-
 isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79L) Bacillus subtilis
0.0344
-
 geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79L) Bacillus subtilis
0.0568
-
 geranylgeranyl diphosphate pH 8.5, 37°C, mutant enzyme subunit-I(wild-type)/subunit-II(A79F) Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P31112 and P31114 P31112: subunit 1, P31114: subunit 2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate
-
 Bacillus subtilis 3 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
geranyl diphosphate + 4 isopentenyl diphosphate geranyl diphosphate is a poor substrate Bacillus subtilis 4 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
geranylgeranyl diphosphate + 2 isopentenyl diphosphate
-
 Bacillus subtilis 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
HepPP synthase
-
 Bacillus subtilis