Literature summary for 2.5.1.30 extracted from

Nagaki, M.; Kimura, K.; Kimura, H.; Maki, Y.; Goto, E.; Nishino, T.; Koyama, T.
Artificial substrates of medium-chain elongating enzymes, hexaprenyl- and heptaprenyl diphosphate synthases (2001), Bioorg. Med. Chem. Lett., 11, 2157-2159.

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Protein Variants

Protein Variants	Comment	Organism
additional information	the hybrid-type combination of component I (Bacillus subtilis) and component II' (Bacillus stearothermophilus) gives distinct prenyltransferase activity. The hybrid-type enzyme catalyzes the synthesis of heptaprenyl diphosphate and shows moderate heat stability, which is between those of the natural enzymes from Bacillus subtilis and Bacillus stearothermophilus	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P31112 and P31114	P31112: subunit 1, P31114: subunit 2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + 3-ethylbut-3-enyl diphosphate	-	Bacillus subtilis	diphosphate + (all-E)-3-ethyl-7,11,15-trimethylhexadeca-2,6,10,14-tetraenyl diphosphate	-	?
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate	-	Bacillus subtilis	4 diphosphate + all-trans-heptaprenyl diphosphate	-	?
(2E,6E)-farnesyl diphosphate + but-3-enyl diphosphate	-	Bacillus subtilis	diphosphate + (E)-norgeranylgeranyl diphosphate	-	?
additional information	norfarnesyl diphosphate is not accepted as substrate	Bacillus subtilis	?	-	?

Subunits

Subunits	Comment	Organism
More	the hybrid-type combination of component I (Bacillus subtilis) and component II (Bacillus stearothermophilus) gives distinct prenyltransferase activity. The hybrid-type enzyme catalyzes the synthesis of heptaprenyl diphosphate and shows moderate heat stability, which is between those of the natural enzymes from Bacillus subtilis and Bacillus stearothermophilus. There is no possibility of forming a hybrid between the heptaprenyl and hexaprenyl diphosphate synthases	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
HepPS	-	Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	the hybrid-type combination of component I (Bacillus subtilis) and component II (Bacillus stearothermophilus) shows moderate heat stability, which is between those of the natural enzymes from Bacillus subtilis and Bacillus stearothermophilus. There is no possibility of forming a hybrid between the heptaprenyl and hexaprenyl diphosphate synthases	Bacillus subtilis

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Release 2023.1 (January 2023)