Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, mutant enzymes | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D73A | mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type | Bacillus subtilis |
D97A | reaction products have marked differences in chain length distribution from the wild-type enzyme. D97A produces larger amounts of shorter chain prenyl diphosphates | Bacillus subtilis |
E128V | mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type | Bacillus subtilis |
K130I | mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type | Bacillus subtilis |
L94S | 9fold lower Vmax values and 7fold higher Km-values for the allylic substrate farnesyl diphosphate compared to wild-type enzyme | Bacillus subtilis |
N127A | mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type | Bacillus subtilis |
T76V | mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type | Bacillus subtilis |
V93G | 16fold lower Vmax values and 10fold higher Km-values for the allylic substrate farnesyl diphosphate compared to wild-type enzyme | Bacillus subtilis |
Y103S | reaction products have marked differences in chain length distribution from the wild-type enzyme. Y103S gives octaprenyl diphosphate (C40) as the final product | Bacillus subtilis |
Y104S | 5fold lower Vmax values and 3fold higher Km-values for the allylic substrate farnesyl diphosphate compared to wild-type enzyme | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0053 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme L107S | Bacillus subtilis | |
0.0056 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme E128V | Bacillus subtilis | |
0.0064 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme D73A | Bacillus subtilis | |
0.0069 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme D97A | Bacillus subtilis | |
0.0072 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, wild-type enzyme | Bacillus subtilis | |
0.0072 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme K130I | Bacillus subtilis | |
0.0072 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme L102S | Bacillus subtilis | |
0.0082 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme N127A | Bacillus subtilis | |
0.0084 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme T76V | Bacillus subtilis | |
0.0098 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme Y103S | Bacillus subtilis | |
0.0103 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme S100G | Bacillus subtilis | |
0.0134 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme K130I | Bacillus subtilis | |
0.0142 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme S100G | Bacillus subtilis | |
0.0156 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme D97A | Bacillus subtilis | |
0.0161 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme D73A | Bacillus subtilis | |
0.0167 | - |
isopentenyl diphosphate | pH 8.5, 37°C, wild-type enzyme | Bacillus subtilis | |
0.0167 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme Y104S | Bacillus subtilis | |
0.0172 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme E128V | Bacillus subtilis | |
0.0175 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme L107S | Bacillus subtilis | |
0.0183 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme T76V | Bacillus subtilis | |
0.0188 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme Y103S | Bacillus subtilis | |
0.0194 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme L94S | Bacillus subtilis | |
0.0194 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme N127A | Bacillus subtilis | |
0.02 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme Y104S | Bacillus subtilis | |
0.0203 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme L102S | Bacillus subtilis | |
0.0226 | - |
isopentenyl diphosphate | pH 8.5, 37°C, mutant enzyme V93G | Bacillus subtilis | |
0.0508 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme L94S | Bacillus subtilis | |
0.0741 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C, mutant enzyme V93G | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P31112 and P31114 | P31112: subunit 1, P31114: subunit 2 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | - |
Bacillus subtilis | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HepPP synthase | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, wild-type and mutant enzymes retain almost original activity after heating | Bacillus subtilis |