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Literature summary for 2.5.1.30 extracted from

  • Zhang, Y.W.; Li, X.Y.; Sugawara, H.; Koyama, T.
    Site-directed mutagenesis of the conserved residues in component I of Bacillus subtilis heptaprenyl diphosphate synthase (1999), Biochemistry, 38, 14638-14643.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, mutant enzymes Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D73A mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type Bacillus subtilis
D97A reaction products have marked differences in chain length distribution from the wild-type enzyme. D97A produces larger amounts of shorter chain prenyl diphosphates Bacillus subtilis
E128V mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type Bacillus subtilis
K130I mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type Bacillus subtilis
L94S 9fold lower Vmax values and 7fold higher Km-values for the allylic substrate farnesyl diphosphate compared to wild-type enzyme Bacillus subtilis
N127A mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type Bacillus subtilis
T76V mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type Bacillus subtilis
V93G 16fold lower Vmax values and 10fold higher Km-values for the allylic substrate farnesyl diphosphate compared to wild-type enzyme Bacillus subtilis
Y103S reaction products have marked differences in chain length distribution from the wild-type enzyme. Y103S gives octaprenyl diphosphate (C40) as the final product Bacillus subtilis
Y104S 5fold lower Vmax values and 3fold higher Km-values for the allylic substrate farnesyl diphosphate compared to wild-type enzyme Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0053
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme L107S Bacillus subtilis
0.0056
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme E128V Bacillus subtilis
0.0064
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme D73A Bacillus subtilis
0.0069
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme D97A Bacillus subtilis
0.0072
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, wild-type enzyme Bacillus subtilis
0.0072
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme K130I Bacillus subtilis
0.0072
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme L102S Bacillus subtilis
0.0082
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme N127A Bacillus subtilis
0.0084
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme T76V Bacillus subtilis
0.0098
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme Y103S Bacillus subtilis
0.0103
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme S100G Bacillus subtilis
0.0134
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme K130I Bacillus subtilis
0.0142
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme S100G Bacillus subtilis
0.0156
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme D97A Bacillus subtilis
0.0161
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme D73A Bacillus subtilis
0.0167
-
isopentenyl diphosphate pH 8.5, 37°C, wild-type enzyme Bacillus subtilis
0.0167
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme Y104S Bacillus subtilis
0.0172
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme E128V Bacillus subtilis
0.0175
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme L107S Bacillus subtilis
0.0183
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme T76V Bacillus subtilis
0.0188
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme Y103S Bacillus subtilis
0.0194
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme L94S Bacillus subtilis
0.0194
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme N127A Bacillus subtilis
0.02
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme Y104S Bacillus subtilis
0.0203
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme L102S Bacillus subtilis
0.0226
-
isopentenyl diphosphate pH 8.5, 37°C, mutant enzyme V93G Bacillus subtilis
0.0508
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme L94S Bacillus subtilis
0.0741
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, mutant enzyme V93G Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P31112 and P31114 P31112: subunit 1, P31114: subunit 2
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate
-
Bacillus subtilis 4 diphosphate + all-trans-heptaprenyl diphosphate
-
?

Synonyms

Synonyms Comment Organism
HepPP synthase
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
30 min, wild-type and mutant enzymes retain almost original activity after heating Bacillus subtilis