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Literature summary for 2.5.1.29 extracted from

  • Ignea, C.; Trikka, F.A.; Nikolaidis, A.K.; Georgantea, P.; Ioannou, E.; Loupassaki, S.; Kefalas, P.; Kanellis, A.K.; Roussis, V.; Makris, A.M.; Kampranis, S.C.
    Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase (2015), Metab. Eng., 27, 65-75.
    View publication on PubMed
Search for more literature for 2.5.1.29

Cloned(Commentary)

Cloned (Comment) Organism
gene erg20 mutants, recombinant expression of N-terminally myc-tagged enzyme, coexpression of the enzyme fused to Cistus creticus 8-hydroxycopalyl diphosphate synthase, CcCLS in Saccharomyces cerevisiae strain AM205 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
F96C site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type Saccharomyces cerevisiae
F96S site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type Saccharomyces cerevisiae
additional information efficient diterpene production in yeast by engineering farnesyl diphosphate synthase Erg20p, EC 2.5.1.10, into a geranylgeranyl diphosphate synthase, overview. Several Erg20p mutants support sclareol yield significantly higher than the yield obtained by overexpression of Cistus creticus GGPPS. A single chromosomally integrated copy of the ERG20(F96C) mutant gene is significantly more efficient than overexpression of a heterologous fungal GGPP synthase in redirecting precursor fluxes towards the diterpene branch Saccharomyces cerevisiae
Y95A site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type Saccharomyces cerevisiae
Y95C/F96H site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type Saccharomyces cerevisiae
Y95L/F96I site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type Saccharomyces cerevisiae
Y95S/F96H site-directed mutagenesis, the mutant shows increased sclareol biosynthesis compared to the wild-type Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae
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 diphosphate + geranylgeranyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12051 gene erg20
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Saccharomyces cerevisiae diphosphate + geranylgeranyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
GGPP synthase
-
 Saccharomyces cerevisiae
GGPPS
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction inefficient substrate channeling between Erg20p and Bts1p results in farnesyl diphosphate accumulation, which in turn leads to feedback inhibition or precursor drain through competing reactions (sterol biosynthesis, dephosphorylation, or other) Saccharomyces cerevisiae
metabolism biosynthesis of sclareol from geranylgeranyl diphosphate, overview Saccharomyces cerevisiae
additional information enzyme structure homology modelling and structure comparisons, overview Saccharomyces cerevisiae
physiological function GGPP synthase catalyzes all three isopentenyl diphosphate additions to dimethylally diphosphate, carrying out in the same active site the reactions catalyzed by both Erg20p (farnesyl diphosphate synthase, EC 2.5.1.10) and Bts1p (geranylgeranyl diphosphate synthase, EC 2.5.1.29) Saccharomyces cerevisiae