Application | Comment | Organism |
---|---|---|
drug development | the enzyme possibly represents an attractive drug target for the development of selective inhibitors aiming the erythrocytic stages of Plasmodium falciparum and development of more potent bisphosphonate-based inhibitors selectivity targeting this key point of the plasmodial isoprenoid metabolism | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
gene PF3D7_1128400, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3+) pLys RIL | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is inhibited by nitrogen-containing bisphosphonates | Plasmodium falciparum | |
risedronate | competitive inhibitor | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Plasmodium falciparum | |
0.00081 | - |
isopentenyl diphosphate | pH 7.5, 37°C, recombinant enzyme, with geranyl diphosphate | Plasmodium falciparum | |
0.002 | - |
isopentenyl diphosphate | pH 7.5, 37°C, recombinant enzyme, with dimethylallyl diphosphate | Plasmodium falciparum | |
0.0021 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C, recombinant enzyme, with isopentenyl diphosphate | Plasmodium falciparum | |
0.0024 | - |
isopentenyl diphosphate | pH 7.5, 37°C, recombinant enzyme, with (2E,6E)-farnesyl diphosphate | Plasmodium falciparum | |
0.0078 | - |
geranyl diphosphate | pH 7.5, 37°C, recombinant enzyme, with isopentenyl diphosphate | Plasmodium falciparum | |
0.068 | - |
dimethylallyl diphosphate | pH 7.5, 37°C, recombinant enzyme, with isopentenyl diphosphate | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
- |
Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Plasmodium falciparum | a bifunctional enzyme farnesyl diphosphate/geranylgeranyl diphosphate synthase exists in Plasmodium falciparum | ? | - |
? | |
additional information | Plasmodium falciparum clone 3D7 | a bifunctional enzyme farnesyl diphosphate/geranylgeranyl diphosphate synthase exists in Plasmodium falciparum | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8II79 | gene PF3D7_1128400 | - |
Plasmodium falciparum clone 3D7 | Q8II79 | gene PF3D7_1128400 | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3+) pLys RIL by glutathione affinity chromatography to homogeneity | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the enzyme is constitutively expressed in all stages during the asexual intra-erythrocytic cycle of Plasmodium falciparum, overview | Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Plasmodium falciparum | diphosphate + geranylgeranyl diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Plasmodium falciparum clone 3D7 | diphosphate + geranylgeranyl diphosphate | - |
? | |
dimethylallyl diphosphate + 2 isopentenyl diphosphate | the enzyme also performs the synthesis of farnesyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, reaction of EC 2.5.1.92 | Plasmodium falciparum | 2 diphosphate + (2Z,6Z)-farnesyl diphosphate | - |
? | |
dimethylallyl diphosphate + 2 isopentenyl diphosphate | the enzyme also performs the synthesis of farnesyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, reaction of EC 2.5.1.92 | Plasmodium falciparum clone 3D7 | 2 diphosphate + (2Z,6Z)-farnesyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | the enzyme also performs the synthesis of farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate, reaction of EC 2.5.1.10 | Plasmodium falciparum | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | the enzyme also performs the synthesis of farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate, reaction of EC 2.5.1.10 | Plasmodium falciparum clone 3D7 | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
additional information | a bifunctional enzyme farnesyl diphosphate/geranylgeranyl diphosphate synthase exists in Plasmodium falciparum | Plasmodium falciparum | ? | - |
? | |
additional information | product identification by thin layer chromatography and mass spectrometry | Plasmodium falciparum | ? | - |
? | |
additional information | a bifunctional enzyme farnesyl diphosphate/geranylgeranyl diphosphate synthase exists in Plasmodium falciparum | Plasmodium falciparum clone 3D7 | ? | - |
? | |
additional information | product identification by thin layer chromatography and mass spectrometry | Plasmodium falciparum clone 3D7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, about, recombinant GST-tagged enzyme, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
farnesyl diphosphate/geranylgeranyl diphosphate synthase | - |
Plasmodium falciparum |
More | cf. EC 2.5.1.92 and EC 2.5.1.10 | Plasmodium falciparum |
PfFPPS | - |
Plasmodium falciparum |
PfGGPPS | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.5 | assay at | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0013 | - |
pH 7.5, 37°C, recombinant enzyme, versus FPP/IPP | Plasmodium falciparum | risedronate | |
0.01 | - |
pH 7.5, 37°C, recombinant enzyme, versus GPP/IPP | Plasmodium falciparum | risedronate |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is a key enzyme in the metabolism of virtually all isoprenoids and it interconnects the 5-carbon moiety isoprenoid synthesis with the mid- or long-chained compound synthesis. The synthesis of farnesyl diphosphate and geranylgeranyl diphosphate is required for the biosynthesis of ubiquinone, dolichol, carotenoids, menaquinone, tocopherol, and protein prenylation | Plasmodium falciparum |