Cloned (Comment) | Organism |
---|---|
gene GGPS1, genotyping, subcloning in Escherichia coli, expression of GFP-tagged enzyme in wild-type Arabidopsis thaliana plants via Agrobacterium tumefaciens (GV3101) transfection by floral dipping, semi-quantitative RT-PCR enzyme expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an F2 mapping population is established by crossing ggps1-1 (Col ecotype) with the LER ecotype of Arabidopsis thaliana, analysis of sequence polymorphisms in 209 F2 recombinant lines homozygous for the ggps1-1 variegated phenotype place the mutation in a 63-kb region on chromosome 4 contained on bacterial artificial chromosome AP22. Phenotype analysis of two lines with T-DNA insertions in the coding region of At4g36810 (Salk_015098 and Salk_085914), and mutant ggps1-1 phenotype, detailed overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | GGPS1 is the major plastid-localized geranylgeranyl diphosphate synthase isozyme in Arabidopsis thaliana | Arabidopsis thaliana | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P34802 | gene GGPS1 | - |
Arabidopsis thaliana Col-0 | P34802 | gene GGPS1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Synonyms | Comment | Organism |
---|---|---|
geranylgeranyl diphosphate synthase 1 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the two T-DNA insertion mutant alleles, ggps1-2 and ggps1-3, result in seedling lethal albino and embryo-lethal phenotypes, respectively. Mutations in geranylgeranyl diphosphate synthase 1 affect chloroplast development in Arabidopsis thaliana. Temperature-sensitive leaf variegation mutant ggps1-1 is caused by a point mutation. Total chlorophyll and carotenoid levels are reduced in ggps1-1 white tissues as compared with green tissues. The ggps1-1 green sector photosynthetic rate is not elevated relative to wild-type tissues. Chloroplast development in green sectors of mutated leaves appear normal, whereas cells in white sectors contain abnormal plastids with numerous electron translucent bodies and poorly developed internal membranes. Mutant ggps1-1 phenotype, detailed overview | Arabidopsis thaliana |
physiological function | geranylgeranyl diphosphate synthase is a key enzyme in the isoprenoid biosynthetic pathway that catalyzes the formation of geranylgeranyl diphosphate, a precursor molecule to several biochemical pathways including those that lead into the biosynthesis of carotenoids and abscisic acid, prenyllipids such as the chlorophylls, and diterpenes such as gibberellic acid, in chlorplasts. GGPS1 is an essential key gene in the chlorophyll biosynthetic pathway | Arabidopsis thaliana |