Cloned (Comment) | Organism |
---|---|
the histidine-tagged deletion mutants (from the carboxyl terminus) (DELTA-4, DELTA-8, DELTA-12 and DELTa-16) are overexpressed in Escherichia coli BL21 (DE3) and purified in a stable form by nickel affinity chromatography except for one mutant DELTA-16 | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the farnesyl-transferring activities of wild-type GGPS, DELTA-4, DELTA-8 and DELTA-12 carboxyterminal deletion mutants are relatively in a ratio of 1.0, 0.84, 0.26 and 0.0015. Each Km value of the four recombinants are estimated to be 0.00071, 0.002, 0.0028 and 0.055 mM for farnesyl diphosphate and to be 0.0029, 0.0051, 0.056 and above 0.1 mM for isopentenyl diphosphate, respectively. Allylic substrate specificities of these recombinants are estimated by quantitative analysis of the products, revealing that DELTA-8 and DELTA-12 mutants lack the ability to accept dimethylallyl and geranyl diphosphates compared to wild-type GGPS and DELTA-4 mutant | Rattus norvegicus |
General Stability | Organism |
---|---|
KMFTEENE residing on the carboxyl-terminal sequence of GGPS stabilizes the active region involved in the substrate binding and catalysis | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00071 | - |
trans,trans-farnesyl diphosphate | pH 7.0, 37°C, wild-type enzyme | Rattus norvegicus | |
0.002 | - |
trans,trans-farnesyl diphosphate | pH 7.0, 37°C, carboxy-terminal deletion mutant DELTA-4 | Rattus norvegicus | |
0.0028 | - |
trans,trans-farnesyl diphosphate | pH 7.0, 37°C, carboxy-terminal deletion mutant DELTA-8 | Rattus norvegicus | |
0.0029 | - |
isopentenyl diphosphate | pH 7.0, 37°C, wild-type enzyme | Rattus norvegicus | |
0.0051 | - |
isopentenyl diphosphate | pH 7.0, 37°C, carboxy-terminal deletion mutant DELTA-4 | Rattus norvegicus | |
0.055 | - |
trans,trans-farnesyl diphosphate | pH 7.0, 37°C, carboxy-terminal deletion mutant DELTA-12 | Rattus norvegicus | |
0.056 | - |
isopentenyl diphosphate | pH 7.0, 37°C, carboxy-terminal deletion mutant DELTA-8 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the histidine-tagged deletion mutants (from the carboxyl terminus) (DELTA-4, DELTA-8, DELTA-12 and DELTa-16) are overexpressed in Escherichia coli BL21 (DE3) and purified in a stable form by nickel affinity chromatography except for one mutant DELTA-16 | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000176 | - |
carboxy-terminal deletion mutant DELTA-8 | Rattus norvegicus |
0.0306 | - |
carboxy-terminal deletion mutant DELTA-8 | Rattus norvegicus |
0.0983 | - |
carboxy-terminal deletion mutant DELTA-4 | Rattus norvegicus |
0.117 | - |
wild-type enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans,trans-farnesyl diphosphate + isopentenyl diphosphate | - |
Rattus norvegicus | diphosphate + geranylgeranyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
geranylgeranyl diphosphate synthase | - |
Rattus norvegicus |
GGPS | - |
Rattus norvegicus |