Cloned (Comment) | Organism |
---|---|
a short and a long clone, which differ in the substrate specificity, DNA and amino acid sequence determination and analysis, genetic organization, functional complementation of an Escherichia coli strain carrying an Erwinia uredovora gene cluster encoding all pathway enzymes needed for carotenoid biosynthesis, except for GGPPS, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans,trans-farnesyl diphosphate + isopentenyl diphosphate | Zea mays | - |
diphosphate + geranylgeranyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | Q548K3 | cv. B73 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the bifunctional enzyme is encoded by two clones of different lengths, exhibiting different preferences in farnesyl diphosphate synthesis and geranylgeranyl diphosphate synthesis, overview | Zea mays | ? | - |
? | |
trans,trans-farnesyl diphosphate + isopentenyl diphosphate | - |
Zea mays | diphosphate + geranylgeranyl diphosphate | - |
? | |
trans,trans-farnesyl diphosphate + isopentenyl diphosphate | the N-terminal sequence affects the ratio of FPP to GGPP | Zea mays | diphosphate + geranylgeranyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
geranylgeranyl pyrophosphate synthase | - |
Zea mays |
GGPPS | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | activity range | Zea mays |