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Literature summary for 2.5.1.29 extracted from

  • Cervantes-Cervantes, M.; Gallagher, C.E.; Zhu, C.; Wurtzel, E.T.
    Maize cDNAs expressed in endosperm encode functional farnesyl diphosphate synthase with geranylgeranyl diphosphate synthase activity (2006), Plant Physiol., 141, 220-231.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
a short and a long clone, which differ in the substrate specificity, DNA and amino acid sequence determination and analysis, genetic organization, functional complementation of an Escherichia coli strain carrying an Erwinia uredovora gene cluster encoding all pathway enzymes needed for carotenoid biosynthesis, except for GGPPS, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans,trans-farnesyl diphosphate + isopentenyl diphosphate Zea mays
-
diphosphate + geranylgeranyl diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Zea mays Q548K3 cv. B73
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme is encoded by two clones of different lengths, exhibiting different preferences in farnesyl diphosphate synthesis and geranylgeranyl diphosphate synthesis, overview Zea mays ?
-
?
trans,trans-farnesyl diphosphate + isopentenyl diphosphate
-
Zea mays diphosphate + geranylgeranyl diphosphate
-
?
trans,trans-farnesyl diphosphate + isopentenyl diphosphate the N-terminal sequence affects the ratio of FPP to GGPP Zea mays diphosphate + geranylgeranyl diphosphate
-
?

Synonyms

Synonyms Comment Organism
geranylgeranyl pyrophosphate synthase
-
Zea mays
GGPPS
-
Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Zea mays

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10.5 activity range Zea mays