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Literature summary for 2.5.1.22 extracted from

  • Zhang, Z.; Zheng, Y.; Petukh, M.; Pegg, A.; Ikeguchi, Y.; Alexov, E.
    Enhancing human spermine synthase activity by engineered mutations (2013), PLoS Comput. Biol., 9, e1002924.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL-1 Blue cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
S165D/L175E/T178H/C206R the mutant shows increased activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 S-adenosyl 3-(methylthio)propylamine wild type enzyme, at pH 7.5 and 37°C Homo sapiens
0.0009
-
 S-adenosyl 3-(methylthio)propylamine mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C Homo sapiens
0.6
-
 spermidine mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C Homo sapiens
0.8
-
 spermidine wild type enzyme, at pH 7.5 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl 3-(methylthio)propylamine + spermidine Homo sapiens
-
 S-methyl-5'-thioadenosine + spermine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON affinity resin column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
227
-
 mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C Homo sapiens
2425
-
 wild type enzyme, at pH 7.5 and 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl 3-(methylthio)propylamine + spermidine
-
 Homo sapiens S-methyl-5'-thioadenosine + spermine
-
 ?

Synonyms

Synonyms Comment Organism
SMS
-
 Homo sapiens