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Literature summary for 2.5.1.22 extracted from
- Purification of spermidine aminopropyltransferase (spermine synthase) from bovine brain (1983), Methods Enzymol., 94, 276-279.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-methylthioadenosine | strong | Bos taurus |  |
| additional information | not inhibitory: decarboxylated adenosylmethionine | Bos taurus | |
| N-ethylmaleimide | in the absence of sulfhydryl compounds | Bos taurus | |
| PCMB | in the absence of sulfhydryl compounds | Bos taurus | |
| putrescine | weak | Bos taurus | |
| spermine | product inhibition, weak | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
S-adenosylmethioninamine | - |
Bos taurus | |
| 0.006 | - |
spermidine | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, SDS-PAGE | Bos taurus |
| 88000 | 90000 | brain, gel filtration, sedimentation equilibrium centrifugation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.396 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, in buffer containing 0.3 M NaCl and 1 mM spermidine, at least 6 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | S-adenosylmethionine cannot act as aminopropyl donor | Bos taurus | ? | - |
? | |
| S-adenosylmethioninamine + spermidine | strictly specific for spermidine | Bos taurus | 5'-methylthioadenosine + spermine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bos taurus |
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Release 2023.1 (January 2023)
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