Cloned (Comment) | Organism |
---|---|
gene WsSQS, generation of T0 Withania somnifera plants overexpressing squalene synthase by Agrobacterium tumefaciens strain GV2260 mediated transformation using high-expression pCAMBIA 1301 vector under the control of the constitutive CaMV 35S promoter, quantitative RT-PCR enzyme expression analysis. Accumulation of SQS mRNA in the leaves and roots, and less in stems of transgenic plants, as well as tissue-specific accumulation of withanolides. Withaferin A and Withanolide A increase up to 2.0-2.5fold whereas the concentration of Withanolide B and Withanone reach only 1.2 and 1.3fold, respectively | Withania somnifera |
Protein Variants | Comment | Organism |
---|---|---|
additional information | total withanolide content of enzyme overexpressing plants, WsSQS T0 transformed plants, and tissue distribution, overview | Withania somnifera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | a membrane associated enzyme | Withania somnifera | 16020 | - |
microsome | - |
Withania somnifera | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, SDS-PAGE | Withania somnifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | Withania somnifera | - |
squalene + 2 diphosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Withania somnifera | H9L9T8 | gene WsSQS | - |
Purification (Comment) | Organism |
---|---|
partially purified recombinant enzyme in microsomes is further purified by ultracentrifugation | Withania somnifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Withania somnifera | - |
additional information | the age and type of donor tissue play crucial roles in the biochemistry of subsequent cultures lines. In vitro cultures have differential capacity to synthesize secondary metabolites depending on the morphological nature of type of explant utilized | Withania somnifera | - |
root | - |
Withania somnifera | - |
stem | - |
Withania somnifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ | - |
Withania somnifera | squalene + 2 diphosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Withania somnifera |
Synonyms | Comment | Organism |
---|---|---|
SQS | - |
Withania somnifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Withania somnifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Withania somnifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Withania somnifera |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme overexpression in transgenic Withania somnifera plants affects the shoot elongation and multiplication, phenotype, overview | Withania somnifera |
metabolism | enzyme SQS operates at a branch point of the withanolide biosynthetic pathway regulating the metabolic flux and catalyzes the first committed step leading to the synthesis of different withanolides | Withania somnifera |