Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NADPH | maximal activity at 2 mM | Thermosynechococcus vestitus | |
Tween 80 | maximal activity at 2% v/v | Thermosynechococcus vestitus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, His-tagged protein found exclusively in inclusion bodies | Thermosynechococcus vestitus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
zaragozic acid A | pH 7.2, 37°C | Thermosynechococcus vestitus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00097 | - |
farnesyl diphosphate | pH 7.2, 37°C | Thermosynechococcus vestitus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermosynechococcus vestitus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39900 | - |
x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated | Thermosynechococcus vestitus |
41886 | - |
x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated | Thermosynechococcus vestitus |
42000 | - |
x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated | Thermosynechococcus vestitus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein found exclusively in inclusion bodies. Solubilization in presence of glycerol | Thermosynechococcus vestitus |
Storage Stability | Organism |
---|---|
-80°C, stable for at least 6 months | Thermosynechococcus vestitus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 farnesyl diphosphate | - |
Thermosynechococcus vestitus | presqualene diphosphate + diphosphate | in absence of NADPH, formation of presqualene diphosphate | ? | |
farnesyl diphosphate + NADPH + H+ | - |
Thermosynechococcus vestitus | squalene + diphosphate + NADP+ | sole product | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE, x * 41886, electrospray mass spectrometry, x * 39900, calculated | Thermosynechococcus vestitus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermosynechococcus vestitus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.74 | - |
farnesyl diphosphate | - |
Thermosynechococcus vestitus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Thermosynechococcus vestitus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000955 | - |
pH 7.2, 37°C | Thermosynechococcus vestitus | zaragozic acid A |