Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell associated | - |
Cyprinus carpio | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Cyprinus carpio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyprinus carpio | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by ammonium precipitation, CM-cellulose column, DEAE-cellulose column, Cibacron blue Sepharose column, Sephacryl S-300 column, Superdex-200 and Superdex-75 column | Cyprinus carpio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cyprinus carpio | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1'-methylthiamine + aniline | 22% of activity with thiamine | Cyprinus carpio | 4-methyl-5-(2-hydroxyethyl)-thiazole + 4-amino-5-(anilinomethyl)-1,2-dimethylpyrimidin-1-ium | - |
? | |
2'-ethylthiamine + aniline | 63% of activity with thiamine | Cyprinus carpio | 4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-ethylpyrimidin-4-amine | - |
? | |
2-(1-hydroxyethyl)thiamine + aniline | 92% of activity with thiamine | Cyprinus carpio | 4-methyl-2-(1-hydroxyethyl)-5-(2-hydroxyethyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine | - |
? | |
5-chloroethylthiamine + aniline | 67% of activity with thiamine | Cyprinus carpio | 4-methyl-5-chloroethyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine | - |
? | |
5-nor-thiamine + aniline | 88% of activity with thiamine | Cyprinus carpio | 4-methyl-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine | - |
? | |
additional information | no activity with 4'-deaminothiamine and 4'-oxythiamine suggest that 4'-amino group is essential for enzyme activity | Cyprinus carpio | ? | - |
? | |
O-(beta-D-galactosyl)thiamine + aniline | 63% of activity with thiamine | Cyprinus carpio | 4-methyl-5-(2-hydroxyethyl-O-beta-D-galactosyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine | - |
? | |
O-benzoylthiamine + aniline | 91% of activity with thiamine | Cyprinus carpio | 2-(4-methyl-1,3-thiazol-5-yl)ethyl benzoate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine | - |
? | |
O-S-diacetylthiamine + aniline | 50% of activity with thiamine | Cyprinus carpio | 1-acetyl-4-methyl-5-(2-hydroxyethyl-O-acetyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine | - |
? | |
O-succinylthiamine + aniline | 83% of activity with thiamine | Cyprinus carpio | 4-methyl-5-(2-hydroxyethyl-O-succinyl)-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine | - |
? | |
pyrithiamine + aniline | 72% of activity with thiamine | Cyprinus carpio | 2-(2-methylpyridin-3-yl)ethanol + 5-(anilinomethyl)-2-methylpyrimidin-4-amine | - |
? | |
tetrahydrothiamine + aniline | 84% of activity with thiamine | Cyprinus carpio | ? | - |
? | |
thiamine + 2-mercaptoethanol | 63% of activity with aniline | Cyprinus carpio | ? | - |
? | |
thiamine + aniline | taken as 100% of activity of purified enzyme | Cyprinus carpio | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + imidazole | 24% of activity with aniline | Cyprinus carpio | 4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine | - |
? | |
thiamine + L-cysteine | 50% of activity with aniline | Cyprinus carpio | 4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid | - |
? | |
thiamine + pyridine | best substrate with aniline | Cyprinus carpio | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine disulfide + aniline | 26% of activity with thiamine | Cyprinus carpio | ? | - |
? | |
thiamine phosphate + aniline | 64% of activity with thiamine | Cyprinus carpio | 2-(4-methyl-1,3-thiazol-5-yl)ethyl dihydrogenphosphate + 5-(anilinomethyl)-2-methylpyrimidin-4-amine | - |
? | |
thiothiamine + aniline | 12% of activity with thiamine | Cyprinus carpio | 4-methyl-5-(2-hydroxyethyl)-2-thio-thiazole + 5-(anilinomethyl)-2-methylpyrimidin-4-amine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Cyprinus carpio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Cyprinus carpio |