Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | similar effects as pyridine at 10 mM probably due to its action as secondary substrate | Paenibacillus thiaminolyticus | |
4,5-dimethylthiazole | reactivation at 10 mM | Paenibacillus thiaminolyticus | |
5-(2-chloroethyl)-4-methylthiazole | reactivation at 10 mM | Paenibacillus thiaminolyticus | |
5-(2-hydroxyethyl)-4-methylthiazole | reactivation at 10 mM | Paenibacillus thiaminolyticus | |
aniline | reactivation of enzyme at 2 mM | Paenibacillus thiaminolyticus | |
dithiothreitol | similar effects as pyridine at 10 mM probably due to its action as secondary substrate | Paenibacillus thiaminolyticus | |
Pyridine | at 25 mM restores activity inhibited by heteropyrimidine at 0.006 mM | Paenibacillus thiaminolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aniline | inactivation at 0.006 mM; reactivation at 2 mM | Paenibacillus thiaminolyticus | |
Dimethialium | inactivation at 0.006 mM | Paenibacillus thiaminolyticus | |
Heteropyrithiamine | inactivation at 0.006 mM that is restored by pyridine at 25 mM and inhibition of restoration of activity by pyridine at 1 mM | Paenibacillus thiaminolyticus | |
additional information | primary substrates inactivate the enzyme at 0.006 mM, the lost activity can be restored by incubation at 37°C for 60 min after dialysis or by incubation with secondary substrates or acceptor bases, each substrate at high concentrations abolishes the inactivation of the other substrate | Paenibacillus thiaminolyticus | |
p-chloromercuribenzoate | 50% of inhibition at 0.2 mM and inhibits reactivation by pyridine | Paenibacillus thiaminolyticus | |
thiamine | inactivation at 0.006 mM | Paenibacillus thiaminolyticus | |
thiamine diphosphate | inactivation at 0.006 mM | Paenibacillus thiaminolyticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paenibacillus thiaminolyticus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus thiaminolyticus | - |
- |
- |
Paenibacillus thiaminolyticus YUSM I00I | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Paenibacillus thiaminolyticus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | primary substrates inactivate the enzyme at 0.006 mM and secondary substrates or acceptor bases reactivate the enzyme at 10 mM, each substrate at high concentrations eliminates the inactivation of the other substrate | Paenibacillus thiaminolyticus | ? | - |
? | |
additional information | primary substrates inactivate the enzyme at 0.006 mM and secondary substrates or acceptor bases reactivate the enzyme at 10 mM, each substrate at high concentrations eliminates the inactivation of the other substrate | Paenibacillus thiaminolyticus YUSM I00I | ? | - |
? | |
thiamine + aniline | - |
Paenibacillus thiaminolyticus | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + aniline | - |
Paenibacillus thiaminolyticus YUSM I00I | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + pyridine | - |
Paenibacillus thiaminolyticus | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
thiamine + pyridine | - |
Paenibacillus thiaminolyticus YUSM I00I | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Paenibacillus thiaminolyticus |
37 | - |
after dialysis, 60 min of incubation restore 90% of activity inhibited by thiamine | Paenibacillus thiaminolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
0.15 M citrate-phosphate buffer | Paenibacillus thiaminolyticus |