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Literature summary for 2.5.1.19 extracted from

  • Zhou, C.; Tian, Y.; Xu, Z.; Zhao, W.; Chen, C.; Bao, W.; Bian, L.; Cai, R.; Wu, A.
    Identification of a new gene encoding 5-enolpyruvylshikimate-3-phosphate synthase using genomic library construction strategy (2012), Mol. Biol. Rep., 39, 10939-47.
    View publication on PubMed

Application

Application Comment Organism
agriculture transgenic Arabidopsis thaliana plants expressing the enzyme from Pseudomonas fluorescens are more resistant to glyphosate exposure compared with wild-type Pseudomonas fluorescens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
glyphosate
-
Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.275
-
3-phosphoshikimate pH 7.0, 28°C Pseudomonas fluorescens
0.29
-
phosphoenolpyruvate pH 7.0, 28°C Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46960
-
x * 46960, calculated Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens D7RH58
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Pseudomonas fluorescens phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 46960, calculated Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.2
-
phosphoenolpyruvate pH 7.0, 28°C Pseudomonas fluorescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0554
-
glyphosate pH 7.0, 28°C Pseudomonas fluorescens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.432
-
pH 7.0, 28°C Pseudomonas fluorescens glyphosate