Application | Comment | Organism |
---|---|---|
agriculture | transgenic Arabidopsis thaliana plants expressing the enzyme from Halothermothrix orenii are more resistant to glyphosate exposure than transgenic plants expressing the Escherichia coli enzyme and control plants | Halothermothrix orenii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Halothermothrix orenii |
Protein Variants | Comment | Organism |
---|---|---|
T355S | mutation results in a catalytically more efficient glyphosate-resistant enzyme | Halothermothrix orenii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyphosate | - |
Halothermothrix orenii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.241 | - |
3-phosphoshikimate | mutant T355S, pH 7.0, 28°C | Halothermothrix orenii | |
0.286 | - |
3-phosphoshikimate | wild-type, pH 7.0, 28°C | Halothermothrix orenii | |
0.292 | - |
phosphoenolpyruvate | mutant T355S, pH 7.0, 28°C | Halothermothrix orenii | |
0.316 | - |
phosphoenolpyruvate | wild-type, pH 7.0, 28°C | Halothermothrix orenii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46020 | - |
x * 46020, calculated | Halothermothrix orenii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halothermothrix orenii | B8CWX4 | - |
- |
Halothermothrix orenii H 168 | B8CWX4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Halothermothrix orenii | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Halothermothrix orenii H 168 | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46020, calculated | Halothermothrix orenii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0446 | - |
glyphosate | wild-type, pH 7.0, 28°C | Halothermothrix orenii | |
0.0512 | - |
glyphosate | mutant T355S, pH 7.0, 28°C | Halothermothrix orenii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.328 | - |
wild-type, pH 7.0, 28°C | Halothermothrix orenii | glyphosate | |
0.398 | - |
mutant T355S, pH 7.0, 28°C | Halothermothrix orenii | glyphosate |