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Literature summary for 2.5.1.19 extracted from
- A novel RPMXR motif among class II 5-enolpyruvylshikimate-3-phosphate synthases is required for enzymatic activity and glyphosate resistance (2009), J. Biotechnol., 144, 330-336.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed as a His-tagged fusion protein | Pseudomonas stutzeri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| M129W | no enzymatic activity | Pseudomonas stutzeri |
| N130D | in a growth complementation assay no difference to wild-type | Pseudomonas stutzeri |
| N130G | in a growth complementation assay no difference to wild-type | Pseudomonas stutzeri |
| N130H | in a growth complementation assay no difference to wild-type | Pseudomonas stutzeri |
| N130L | in a growth complementation assay no difference to wild-type | Pseudomonas stutzeri |
| N130P | partial impaiment in growth complementation assay | Pseudomonas stutzeri |
| N130S | in a growth complementation assay no difference to wild-type, Km (phosphoenolpyruvate): 0.032 mM | Pseudomonas stutzeri |
| N130W | partial impaiment in growth complementation assay, mutant shows a significant decrease in resistance to glyphosate, Km (phosphoenolpyruvate): 0.025 mM | Pseudomonas stutzeri |
| P128R | no enzymatic activity | Pseudomonas stutzeri |
| R127E | no enzymatic activity | Pseudomonas stutzeri |
| R127K | no enzymatic activity | Pseudomonas stutzeri |
| R131E | no enzymatic activity | Pseudomonas stutzeri |
| R131K | no enzymatic activity | Pseudomonas stutzeri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
phosphoenolpyruvate | mutant N130W | Pseudomonas stutzeri |  |
| 0.032 | - |
phosphoenolpyruvate | wild-type | Pseudomonas stutzeri | |
| 0.035 | - |
phosphoenolpyruvate | mutant N130S | Pseudomonas stutzeri | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | A4VLZ3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + 3-phosphoshikimate | - |
Pseudomonas stutzeri | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AroA | - |
Pseudomonas stutzeri |
| EPSP synthase | - |
Pseudomonas stutzeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas stutzeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Pseudomonas stutzeri |
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Release 2023.1 (January 2023)
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