Literature summary for 2.5.1.19 extracted from

Marques, M.R.; Vaso, A.; Neto, J.R.; Fossey, M.A.; Oliveira, J.S.; Basso, L.A.; dos Santos, D.S.; de Azevedo Junior, W.F.; Palma, M.S.
Dynamics of glyphosate-induced conformational changes of Mycobacterium tuberculosis 5-enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) determined by hydrogen-deuterium exchange and electrospray mass spectrometry (2008), Biochemistry, 47, 7509-7522.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
restrained-based, molecular modelling; open conformation model of apoenzyme generated based on coordinates from PDB: 2BJB (MtEPSPS), closed conformation model of enzyme/3-phosphoshikimate/glyphosate complex generated based on PDB: 1G6S	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
glyphosate	Kd: 350 micromolar (pH 6.9, 25°C), binding induces conformational change that leads to increase in alpha helix (40% to 57%) and decrease in beta-sheet (30% to 23%) content (revealed by circular dichroism spectroscopy) and prevents access of solvent to core molecule (revealed by hydrogen/deuterium exchange, peptic digestion and LC-ESI-MS)	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46430	-	by ESI-MS	Mycobacterium tuberculosis
46430	-	deduced from primary structure	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WPY5	-	-
Mycobacterium tuberculosis H37Rv	P9WPY5	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
pellet from 80% (w/v) ammonium sulfate solution, resolubilization and dialysis against 5 mM sodium phosphate, pH 6.9	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
Mt EPSPS	-	Mycobacterium tuberculosis
Mycobacterium tuberculosis 5-enolpyruvylshikimate-3-phosphate synthase	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)