Application | Comment | Organism |
---|---|---|
biotechnology | glyphosate tolerance in bacteria | Dunaliella salina |
Cloned (Comment) | Organism |
---|---|
amplified by 3 and 5 RACE on total RNA according to an EST found in Dunaliella salina cDNA library, cloned into pMD18-T for sequencing, cloned in pGEX-4T-1 for expression as mature protein (lacking N-terminal 70 amino acids long transit peptide) in Escherichia coli JM109 or EPSP synthase gene deleted Escherichia coli strain ER2799 | Dunaliella salina |
expression in Escherichia coli | Dunaliella salina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | putative N-terminal transit peptide consisting of 70 amino acids which is present in other plant EPSP synthases and lacking in the bacterial enzyme | Dunaliella salina | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54600 | - |
deduced from primary structure, 514 amino acids | Dunaliella salina |
73000 | - |
overexpressed mature protein (47000 Da) with GST tag (26000 Da) | Dunaliella salina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Dunaliella salina | sixth enzyme in shikimate pathway, biosynthesis of aromatic amino acids | ? | - |
? | |
additional information | Dunaliella salina 435 | sixth enzyme in shikimate pathway, biosynthesis of aromatic amino acids | ? | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | Dunaliella salina | sixth enzyme in the shikimate pathway. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide lyphosate | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | Dunaliella salina 435 | sixth enzyme in the shikimate pathway. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide lyphosate | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dunaliella salina | A2T9R8 | - |
- |
Dunaliella salina 435 | A2T9R8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | sixth enzyme in shikimate pathway, biosynthesis of aromatic amino acids | Dunaliella salina | ? | - |
? | |
additional information | sixth enzyme in shikimate pathway, biosynthesis of aromatic amino acids | Dunaliella salina 435 | ? | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | sixth enzyme in the shikimate pathway. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide lyphosate | Dunaliella salina | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | sixth enzyme in the shikimate pathway. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide lyphosate | Dunaliella salina 435 | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
shikimate 3-phosphate + phosphoenolpyruvate | activity independent of transit peptide (N-terminal 70 residues) | Dunaliella salina | phosphate + 5-enpolpyruvylshikimate 3-phosphate | growth of transformed Escherichia coli lacking endogenous EPSP synthase on M9 minimal medium by sustaining synthesis of aromatic amino acids through introduced 5-enolpyruvylshikimate 3-phosphate synthesis, leading to 10fold increase in glyphosate tolerance in bacteria compared to controls | ? | |
shikimate 3-phosphate + phosphoenolpyruvate | activity independent of transit peptide (N-terminal 70 residues) | Dunaliella salina 435 | phosphate + 5-enpolpyruvylshikimate 3-phosphate | growth of transformed Escherichia coli lacking endogenous EPSP synthase on M9 minimal medium by sustaining synthesis of aromatic amino acids through introduced 5-enolpyruvylshikimate 3-phosphate synthesis, leading to 10fold increase in glyphosate tolerance in bacteria compared to controls | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54600, cylculated from sequence | Dunaliella salina |
Synonyms | Comment | Organism |
---|---|---|
DsaroA | - |
Dunaliella salina |
Dunaliella salina 5-enolpyruvylshikimate-3-phosphate synthase | - |
Dunaliella salina |
Dunaliella salina EPSP synthase | - |
Dunaliella salina |