Literature summary for 2.5.1.19 extracted from

Mizyed, S.; Wright, J.E.; Byczynski, B.; Berti, P.J.
Identification of the catalytic residues of AroA (Enolpyruvylshikimate 3-phosphate synthase) using partitioning analysis (2003), Biochemistry, 42, 6986-6995.

Search for more literature for 2.5.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of wild-type, His-tagged wild-type and several mutant enzymes in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D313A	0.42% of wild-type activity	Escherichia coli
D313N	5% of wild-type activity	Escherichia coli
D49A	41% of wild-type activity	Escherichia coli
E341A	0.3% of wild-type activity	Escherichia coli
E341Q	10% of wild-type activity	Escherichia coli
H385A	0.08% of wild-type activity	Escherichia coli
K22A	0.7% of wild-type activity	Escherichia coli
K22R	3% of wild-type activity	Escherichia coli
K340A	2.4% of wild-type activity	Escherichia coli
K411A	10.4% of wild-type activity	Escherichia coli
N94A	50% of wild-type activity	Escherichia coli
Q171A	1.7% of wild-type activity	Escherichia coli
R100M	0.2% of wild-type activity	Escherichia coli
R124A	19.6% of wild-type activity	Escherichia coli
R344K	31.7% of wild-type activity	Escherichia coli
R344M	16.3% of wild-type activity	Escherichia coli
R386M	15.8% of wild-type activity	Escherichia coli
Y200F	1% of wild-type activity	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A6D3	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
20	-	recombinant His-tagged wild-type enzyme	Escherichia coli
24	-	recombinant wild-type enzyme	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + 3-phosphoshikimate	-	Escherichia coli	phosphate + 5-enolpyruvylshikimate 3-phosphate	-	r

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Release 2023.1 (January 2023)