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Literature summary for 2.5.1.19 extracted from
- Purificatioin and properties of 5-enolpyruvylshikimate-3-phosphate synthase from dark-grown seedlings of Sorghum bicolor (1988), Plant Physiol., 87, 232-238.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | at about 100 mM | Sorghum bicolor |  |
| F- | stimulation | Sorghum bicolor | |
| NO3- | stimulation at low concentrations, inhibition above 15 mM | Sorghum bicolor | |
| SO42- | slight stimulation, inhibition above 50 mM | Sorghum bicolor | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonium heptamolybdate | 1.1 mM, 50% inhibition, no inhibition below 0.1 mM | Sorghum bicolor | |
| CaCl2 | above 100 mM | Sorghum bicolor | |
| Cu2+ | 5 mM, complete inhibition | Sorghum bicolor | |
| Fe2+ | 5 mM, 42% inhibition | Sorghum bicolor | |
| Fe3+ | 5 mM, 68% inhibition | Sorghum bicolor | |
| MgCl2 | above 100 mM | Sorghum bicolor | |
| Mn2+ | 5 mM, 42% inhibition | Sorghum bicolor | |
| N-phosphonomethylglycine | - |
Sorghum bicolor | |
| Pb2+ | 5 mM, 97% inhibition | Sorghum bicolor | |
| SO42- | inhibition above 50 mM, slight stimulation below | Sorghum bicolor | |
| Zn2+ | 5 mM, complete inhibition | Sorghum bicolor | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
3-phosphoshikimate | - |
Sorghum bicolor | |
| 0.008 | - |
phosphoenolpyruvate | - |
Sorghum bicolor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | stimulation at 20 mM, inhibition above 100 mM | Sorghum bicolor | |
| MgCl2 | stimulation at 20 mM, inhibition above 100 mM | Sorghum bicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
gel filtration | Sorghum bicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sorghum bicolor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose, hydroxyapatite, phenylaggarose, Sephacryl S-200, isozymes I, II and III | Sorghum bicolor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.1 | - |
- |
Sorghum bicolor |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 40% v/v glycerol, at least 1 year, no loss of activity | Sorghum bicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + 3-phosphoshikimate | - |
Sorghum bicolor | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50600-51900, 3 isozymes, SDS-PAGE | Sorghum bicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sorghum bicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
Tris-HCl buffer preferred | Sorghum bicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | approx. half-maximal activity at pH 6.5 and 8.5 | Sorghum bicolor |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00016 | - |
N-phosphonomethylglycine | vs. phosphoenolpyruvate | Sorghum bicolor | |
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