Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4,4'-dichlorodiphenyltrichloroethane | - |
Glycine max | |
alachlor | - |
Glycine max | |
fenvalerate | - |
Glycine max | |
lambda-cyhalothrin | - |
Glycine max | |
spirodichlofen | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0214 | - |
glutathione | with cumene hydroperoxide as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
0.0273 | - |
tert-butyl peroxide | pH and temperature not specified in the publication | Glycine max | |
0.0524 | - |
glutathione | with fluorodifen as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
0.0615 | - |
glutathione | with tert-butyl peroxide as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
0.0771 | - |
glutathione | with 1-chloro-2,4-dinitrobenzene as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
0.0788 | - |
fluorodifen | pH and temperature not specified in the publication | Glycine max | |
0.1033 | - |
1-chloro-2,4-dinitrobenzene | pH and temperature not specified in the publication | Glycine max | |
0.439 | - |
cumene hydroperoxide | pH and temperature not specified in the publication | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1,4-butanediol diglycidyl ether-GSH-Sepharose-CL6B column chromatography | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | - |
Glycine max | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
cumene hydroperoxide + glutathione | - |
Glycine max | ? | - |
? | |
fluorodifen + glutathione | - |
Glycine max | ? | - |
? | |
tert-butyl peroxide + glutathione | - |
Glycine max | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GSTU2-2 | isoenzyme | Glycine max |
tau class glutathione transferase | - |
Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00098 | - |
glutathione | with fluorodifen as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
0.16 | - |
glutathione | with cumene hydroperoxide as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
1.42 | - |
glutathione | with 1-chloro-2,4-dinitrobenzene as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
1.43 | - |
glutathione | with tert-butyl peroxide as cosubstrate, pH and temperature not specified in the publication | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Glycine max |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0048 | - |
pH and temperature not specified in the publication | Glycine max | fenvalerate | |
0.0048 | - |
pH and temperature not specified in the publication | Glycine max | lambda-cyhalothrin | |
0.01 | - |
pH and temperature not specified in the publication | Glycine max | alachlor | |
0.0285 | - |
pH and temperature not specified in the publication | Glycine max | 4,4'-dichlorodiphenyltrichloroethane | |
0.0412 | - |
pH and temperature not specified in the publication | Glycine max | spirodichlofen |
Organism | Comment | Expression |
---|---|---|
Glycine max | isoform GSTU2 expression is strongly induced by 100 mM NaCl | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
fluorodifen | pH and temperature not specified in the publication | Glycine max | |
0.018 | - |
glutathione | with fluorodifen as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
0.37 | - |
cumene hydroperoxide | pH and temperature not specified in the publication | Glycine max | |
7.5 | - |
glutathione | with cumene hydroperoxide as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
13.7 | - |
1-chloro-2,4-dinitrobenzene | pH and temperature not specified in the publication | Glycine max | |
18.4 | - |
glutathione | with 1-chloro-2,4-dinitrobenzene as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
23.2 | - |
glutathione | with tert-butyl peroxide as cosubstrate, pH and temperature not specified in the publication | Glycine max | |
52.3 | - |
tert-butyl peroxide | pH and temperature not specified in the publication | Glycine max |