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Literature summary for 2.5.1.18 extracted from

  • Skopelitou, K.; Muleta, A.W.; Papageorgiou, A.C.; Chronopoulou, E.G.; Pavli, O.; Flemetakis, E.; Skaracis, G.N.; Labrou, N.E.
    Characterization and functional analysis of a recombinant tau class glutathione transferase GmGSTU2-2 from Glycine max (2017), Int. J. Biol. Macromol., 94 B, 802-812.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Glycine max

Inhibitors

Inhibitors Comment Organism Structure
4,4'-dichlorodiphenyltrichloroethane
-
Glycine max
alachlor
-
Glycine max
fenvalerate
-
Glycine max
lambda-cyhalothrin
-
Glycine max
spirodichlofen
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0214
-
glutathione with cumene hydroperoxide as cosubstrate, pH and temperature not specified in the publication Glycine max
0.0273
-
tert-butyl peroxide pH and temperature not specified in the publication Glycine max
0.0524
-
glutathione with fluorodifen as cosubstrate, pH and temperature not specified in the publication Glycine max
0.0615
-
glutathione with tert-butyl peroxide as cosubstrate, pH and temperature not specified in the publication Glycine max
0.0771
-
glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, pH and temperature not specified in the publication Glycine max
0.0788
-
fluorodifen pH and temperature not specified in the publication Glycine max
0.1033
-
1-chloro-2,4-dinitrobenzene pH and temperature not specified in the publication Glycine max
0.439
-
cumene hydroperoxide pH and temperature not specified in the publication Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1,4-butanediol diglycidyl ether-GSH-Sepharose-CL6B column chromatography Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
Glycine max S-(2,4-dinitrophenyl)glutathione + HCl
-
?
cumene hydroperoxide + glutathione
-
Glycine max ?
-
?
fluorodifen + glutathione
-
Glycine max ?
-
?
tert-butyl peroxide + glutathione
-
Glycine max ?
-
?

Synonyms

Synonyms Comment Organism
GSTU2-2 isoenzyme Glycine max
tau class glutathione transferase
-
Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00098
-
glutathione with fluorodifen as cosubstrate, pH and temperature not specified in the publication Glycine max
0.16
-
glutathione with cumene hydroperoxide as cosubstrate, pH and temperature not specified in the publication Glycine max
1.42
-
glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, pH and temperature not specified in the publication Glycine max
1.43
-
glutathione with tert-butyl peroxide as cosubstrate, pH and temperature not specified in the publication Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Glycine max

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0048
-
pH and temperature not specified in the publication Glycine max fenvalerate
0.0048
-
pH and temperature not specified in the publication Glycine max lambda-cyhalothrin
0.01
-
pH and temperature not specified in the publication Glycine max alachlor
0.0285
-
pH and temperature not specified in the publication Glycine max 4,4'-dichlorodiphenyltrichloroethane
0.0412
-
pH and temperature not specified in the publication Glycine max spirodichlofen

Expression

Organism Comment Expression
Glycine max isoform GSTU2 expression is strongly induced by 100 mM NaCl up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0125
-
fluorodifen pH and temperature not specified in the publication Glycine max
0.018
-
glutathione with fluorodifen as cosubstrate, pH and temperature not specified in the publication Glycine max
0.37
-
cumene hydroperoxide pH and temperature not specified in the publication Glycine max
7.5
-
glutathione with cumene hydroperoxide as cosubstrate, pH and temperature not specified in the publication Glycine max
13.7
-
1-chloro-2,4-dinitrobenzene pH and temperature not specified in the publication Glycine max
18.4
-
glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, pH and temperature not specified in the publication Glycine max
23.2
-
glutathione with tert-butyl peroxide as cosubstrate, pH and temperature not specified in the publication Glycine max
52.3
-
tert-butyl peroxide pH and temperature not specified in the publication Glycine max