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Literature summary for 2.5.1.18 extracted from
- Structural modelling and molecular characterization of omega-class glutathione S-transferase 2 from Drosophila melanogaster (2014), Insect Mol. Biol., 23, 357-366.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Drosophila melanogaster |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.39 | - |
dehydroascorbate | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster |  |
| 1.03 | - |
dehydroascorbate | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
| 4.23 | - |
glutathione | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
| 4.33 | - |
glutathione | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Drosophila melanogaster | 5737 | - |
| mitochondrion | - |
Drosophila melanogaster | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28700 | - |
- |
Drosophila melanogaster |
| 29100 | - |
- |
Drosophila melanogaster |
| 30000 | - |
2 * 30000, SDS-PAGE | Drosophila melanogaster |
| 60000 | - |
gel filtration | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | Q9VSL4 | isoform GstO2B | - |
| Drosophila melanogaster | Q9VSL5 | isoform GstO2A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-affinity resin column chromatography | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dehydroascorbate + glutathione | - |
Drosophila melanogaster | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 30000, SDS-PAGE | Drosophila melanogaster |
| homodimer | 2 * 28700, isoform GstO2B, calculated from amino acid sequence | Drosophila melanogaster |
| homodimer | 2 * 29100, isoform GstO2A, calculated from amino acid sequence | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase omega 2 | - |
Drosophila melanogaster |
| GstO2A | isoform | Drosophila melanogaster |
| GstO2B | isoform | Drosophila melanogaster |
| omega-class glutathione S-transferase 2 | - |
Drosophila melanogaster |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Drosophila melanogaster |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 673.7 | - |
dehydroascorbate | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
| 692.8 | - |
glutathione | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
| 905.8 | - |
glutathione | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster | |
| 925.2 | - |
dehydroascorbate | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Drosophila melanogaster |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 164 | - |
glutathione | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
| 209 | - |
glutathione | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster | |
| 654 | - |
dehydroascorbate | isoform GstO2A, at pH 7.6 and 30°C | Drosophila melanogaster | |
| 2370 | - |
dehydroascorbate | isoform GstO2B, at pH 7.6 and 30°C | Drosophila melanogaster | |
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