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Literature summary for 2.5.1.18 extracted from

  • Pandey, T.; Chhetri, G.; Chinta, R.; Kumar, B.; Singh, D.B.; Tripathi, T.; Singh, A.K.
    Functional classification and biochemical characterization of a novel rho class glutathione S-transferase in Synechocystis PCC 6803 (2015), FEBS Open Bio, 5, 1-7.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
Dichloroacetate at pH 7.5 and 30°C Synechocystis sp.
1.5
-
glutathione at pH 7.5 and 30°C Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, and gel filtration Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
Synechocystis sp. S-(2,4-dinitrophenyl)glutathione + HCl
-
?
dichloroacetate + glutathione
-
Synechocystis sp. glyoxylate + HCl + ?
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
rho class glutathione S-transferase
-
Synechocystis sp.
sll1545
-
Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Synechocystis sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 more than 60% activity between 25 and 50°C Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.48
-
glutathione at pH 7.5 and 30°C Synechocystis sp.
82.17
-
Dichloroacetate at pH 7.5 and 30°C Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Synechocystis sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 more than 40% activity between pH 6.0 and 8.5 Synechocystis sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.32
-
glutathione at pH 7.5 and 30°C Synechocystis sp.
574.6
-
Dichloroacetate at pH 7.5 and 30°C Synechocystis sp.