Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
Dichloroacetate | at pH 7.5 and 30°C | Synechocystis sp. | |
1.5 | - |
glutathione | at pH 7.5 and 30°C | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, and gel filtration | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | - |
Synechocystis sp. | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
dichloroacetate + glutathione | - |
Synechocystis sp. | glyoxylate + HCl + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
rho class glutathione S-transferase | - |
Synechocystis sp. |
sll1545 | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Synechocystis sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | more than 60% activity between 25 and 50°C | Synechocystis sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.48 | - |
glutathione | at pH 7.5 and 30°C | Synechocystis sp. | |
82.17 | - |
Dichloroacetate | at pH 7.5 and 30°C | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Synechocystis sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | more than 40% activity between pH 6.0 and 8.5 | Synechocystis sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.32 | - |
glutathione | at pH 7.5 and 30°C | Synechocystis sp. | |
574.6 | - |
Dichloroacetate | at pH 7.5 and 30°C | Synechocystis sp. |