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Literature summary for 2.5.1.18 extracted from

  • Skopelitou, K.; Muleta, A.W.; Papageorgiou, A.C.; Chronopoulou, E.; Labrou, N.E.
    Catalytic features and crystal structure of a tau class glutathione transferase from Glycine max specifically upregulated in response to soybean mosaic virus infections (2015), Biochim. Biophys. Acta, 1854, 166-177.
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Glycine max

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M Bis-Tris, pH 5.6, 22% (w/v) PEG 3350 Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0286
-
 glutathione with cumene hydroperoxide as cosubstrate, at pH 6.5 and 25°C Glycine max
0.0648
-
 glutathione with fluorodifen as cosubstrate, at pH 6.5 and 25°C Glycine max
0.0662
-
 fluorodifen at pH 6.5 and 25°C Glycine max
0.0679
-
 glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, at pH 6.5 and 25°C Glycine max
0.087
-
 glutathione with tert-butyl peroxide as cosubstrate, at pH 6.5 and 25°C Glycine max
0.252
-
 cumene hydroperoxide at pH 6.5 and 25°C Glycine max
0.28
-
 1-chloro-2,4-dinitrobenzene at pH 6.5 and 25°C Glycine max
0.853
-
 tert-butyl peroxide at pH 6.5 and 25°C Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Glycine max 5829
-

Organism

Organism UniProt Comment Textmining
Glycine max Q9FQE8
-
-

Purification (Commentary)

Purification (Comment) Organism
1,4-butanediol diglycidyl ether-GSH-Sepharose-CL6B column chromatography Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
 Glycine max S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
cumene hydroperoxide + glutathione
-
 Glycine max ?
-
 ?
fluorodifen + glutathione
-
 Glycine max ?
-
 ?
tert-butyl peroxide + glutathione
-
 Glycine max ?
-
 ?

Synonyms

Synonyms Comment Organism
GSTU10-10 isoform Glycine max
tau class glutathione transferase
-
 Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
62.4
-
 melting temperature Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
 glutathione with tert-butyl peroxide as cosubstrate, at pH 6.5 and 25°C Glycine max
0.58
-
 glutathione with cumene hydroperoxide as cosubstrate, at pH 6.5 and 25°C Glycine max
0.82
-
 glutathione with fluorodifen as cosubstrate, at pH 6.5 and 25°C Glycine max
2.65
-
 glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, at pH 6.5 and 25°C Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Glycine max

Expression

Organism Comment Expression
Glycine max isoform GSTU10-10 is specifically expressed in response to biotic stress caused by soybean mosaic virus infections up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.33
-
 tert-butyl peroxide at pH 6.5 and 25°C Glycine max
2.3
-
 cumene hydroperoxide at pH 6.5 and 25°C Glycine max
3
-
 glutathione with tert-butyl peroxide as cosubstrate, at pH 6.5 and 25°C Glycine max
9.5
-
 1-chloro-2,4-dinitrobenzene at pH 6.5 and 25°C Glycine max
12.3
-
 fluorodifen at pH 6.5 and 25°C Glycine max
12.7
-
 glutathione with fluorodifen as cosubstrate, at pH 6.5 and 25°C Glycine max
20.3
-
 glutathione with cumene hydroperoxide as cosubstrate, at pH 6.5 and 25°C Glycine max
39.2
-
 glutathione with 1-chloro-2,4-dinitrobenzene as cosubstrate, at pH 6.5 and 25°C Glycine max