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Literature summary for 2.5.1.18 extracted from
- Substrate specificities of two tau class glutathione transferases inducible by 2,4,6-trinitrotoluene in poplar (2015), Biochim. Biophys. Acta, 1850, 1877-1883.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Gold (DE3) cells | Populus trichocarpa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0065 | - |
phenethyl isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa |  |
| 0.0256 | - |
sulforaphane | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.036 | - |
phenethyl isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.038 | - |
cumene hydroperoxide | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.29 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.324 | - |
allyl-isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.759 | - |
allyl-isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 1.69 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 4.853 | - |
cumene hydroperoxide | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 6.178 | - |
sulforaphane | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25378 | - |
- |
Populus trichocarpa |
| 25521 | - |
- |
Populus trichocarpa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus trichocarpa | D2WL48 | - |
- |
| Populus trichocarpa | D2WL57 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography | Populus trichocarpa |
| Ni-Sepharose column chromatography, and gel filtration | Populus trichocarpa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-chloro-2,4-dinitrobenzene + glutathione | - |
Populus trichocarpa | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
| 1-chloro-2,4-dinitrobenzene + glutathione | isoform GSTU45 shows highest activity with 1-chloro-2,4-dinitrobenzene | Populus trichocarpa | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
| 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole + glutathione | - |
Populus trichocarpa | ? | - |
? | |
| allyl-isothiocyanate + glutathione | - |
Populus trichocarpa | ? | - |
? | |
| benzyl-isothiocyanate + glutathione | - |
Populus trichocarpa | ? | - |
? | |
| cumene hydroperoxide + glutathione | - |
Populus trichocarpa | ? | - |
? | |
| additional information | very low or no activity with iodomethane, 1-iodobutane, 1-iodooctane, H2O2, dehydroascorbate, trans-2-hexenal, trans-2-nonenal, 2,4,6-trinitrotoluene, and 2,4-dinitrotoluene | Populus trichocarpa | ? | - |
? | |
| phenethyl isothiocyanate + glutathione | - |
Populus trichocarpa | ? | - |
? | |
| phenethyl isothiocyanate + glutathione | isoform GSTU16 shows highest activity with phenethyl isothiocyanate | Populus trichocarpa | ? | - |
? | |
| propyl-isothiocyanate + glutathione | - |
Populus trichocarpa | ? | - |
? | |
| sulforaphane + glutathione | - |
Populus trichocarpa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25378, isoform GSTU45, calculated from amino acid sequence | Populus trichocarpa |
| ? | x * 25521, isoform GSTU16, calculated from amino acid sequence | Populus trichocarpa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GSTU16 | isoform | Populus trichocarpa |
| GSTU45 | isoform | Populus trichocarpa |
| tau class glutathione transferase | - |
Populus trichocarpa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.073 | - |
sulforaphane | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.14 | - |
phenethyl isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.25 | - |
allyl-isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.27 | - |
cumene hydroperoxide | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.58 | - |
cumene hydroperoxide | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 2.57 | - |
sulforaphane | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 2.89 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 3.6 | - |
allyl-isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 5.3 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 62.9 | - |
phenethyl isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Populus trichocarpa | isoform GSTU16, calculated from amino acid sequence | - |
5.6 |
| Populus trichocarpa | isoform GSTU45, calculated from amino acid sequence | - |
6 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Populus trichocarpa | expression is induced by exposure of poplar tissues to 2,4,6-trinitrotoluene | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
cumene hydroperoxide | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.42 | - |
sulforaphane | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 0.77 | - |
allyl-isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 1.7 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 2.84 | - |
sulforaphane | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 3.75 | - |
phenethyl isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 4.74 | - |
allyl-isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 15.37 | - |
cumene hydroperoxide | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 18.31 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
| 9696 | - |
phenethyl isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
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