Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Gold (DE3) cells | Populus trichocarpa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
phenethyl isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.0256 | - |
sulforaphane | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.036 | - |
phenethyl isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.038 | - |
cumene hydroperoxide | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.29 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.324 | - |
allyl-isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.759 | - |
allyl-isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
1.69 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
4.853 | - |
cumene hydroperoxide | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
6.178 | - |
sulforaphane | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25378 | - |
- |
Populus trichocarpa |
25521 | - |
- |
Populus trichocarpa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus trichocarpa | D2WL48 | - |
- |
Populus trichocarpa | D2WL57 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Populus trichocarpa |
Ni-Sepharose column chromatography, and gel filtration | Populus trichocarpa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | - |
Populus trichocarpa | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
1-chloro-2,4-dinitrobenzene + glutathione | isoform GSTU45 shows highest activity with 1-chloro-2,4-dinitrobenzene | Populus trichocarpa | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
7-chloro-4-nitrobenzo-2-oxa-1,3-diazole + glutathione | - |
Populus trichocarpa | ? | - |
? | |
allyl-isothiocyanate + glutathione | - |
Populus trichocarpa | ? | - |
? | |
benzyl-isothiocyanate + glutathione | - |
Populus trichocarpa | ? | - |
? | |
cumene hydroperoxide + glutathione | - |
Populus trichocarpa | ? | - |
? | |
additional information | very low or no activity with iodomethane, 1-iodobutane, 1-iodooctane, H2O2, dehydroascorbate, trans-2-hexenal, trans-2-nonenal, 2,4,6-trinitrotoluene, and 2,4-dinitrotoluene | Populus trichocarpa | ? | - |
? | |
phenethyl isothiocyanate + glutathione | - |
Populus trichocarpa | ? | - |
? | |
phenethyl isothiocyanate + glutathione | isoform GSTU16 shows highest activity with phenethyl isothiocyanate | Populus trichocarpa | ? | - |
? | |
propyl-isothiocyanate + glutathione | - |
Populus trichocarpa | ? | - |
? | |
sulforaphane + glutathione | - |
Populus trichocarpa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25378, isoform GSTU45, calculated from amino acid sequence | Populus trichocarpa |
? | x * 25521, isoform GSTU16, calculated from amino acid sequence | Populus trichocarpa |
Synonyms | Comment | Organism |
---|---|---|
GSTU16 | isoform | Populus trichocarpa |
GSTU45 | isoform | Populus trichocarpa |
tau class glutathione transferase | - |
Populus trichocarpa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
sulforaphane | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.14 | - |
phenethyl isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.25 | - |
allyl-isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.27 | - |
cumene hydroperoxide | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.58 | - |
cumene hydroperoxide | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
2.57 | - |
sulforaphane | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
2.89 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
3.6 | - |
allyl-isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
5.3 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
62.9 | - |
phenethyl isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Populus trichocarpa | isoform GSTU16, calculated from amino acid sequence | - |
5.6 |
Populus trichocarpa | isoform GSTU45, calculated from amino acid sequence | - |
6 |
Organism | Comment | Expression |
---|---|---|
Populus trichocarpa | expression is induced by exposure of poplar tissues to 2,4,6-trinitrotoluene | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
cumene hydroperoxide | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.42 | - |
sulforaphane | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
0.77 | - |
allyl-isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
1.7 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
2.84 | - |
sulforaphane | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
3.75 | - |
phenethyl isothiocyanate | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
4.74 | - |
allyl-isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
15.37 | - |
cumene hydroperoxide | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa | |
18.31 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTU45, pH not specified in the publication, at 30°C | Populus trichocarpa | |
9696 | - |
phenethyl isothiocyanate | isoform GSTU16, pH not specified in the publication, at 30°C | Populus trichocarpa |