Cloned (Comment) | Organism |
---|---|
isozymes GST A1-1 and GST A4-4, expression in Escherichia coli strain XL-1 Blue, expression of mutant GIMFhelix in Escherichia coli strain Rosetta-gami B(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant GIMFhelix by exchange of active site residues leading to about 300fold enhanced enzyme activity, substrate-activity profile compared to wild-type isozymes, overview. GIMFhelix contains all of the H-site residues identified in isozyme GST A4-4. GIMFhelix showed two orders of magnitude higher catalytic efficiency with long-chain alkenals than isozyme GST A1-1, while the activity with the standard substrate CDNB is one order below that of GST A1-1 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes GST A1-1 and GST A4-4 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozymes GST A1-1 and GST A4-4 and recombinant mutant GIMFhelix from Escherichia coli by glutathione affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methyl-4-nitro-5-[(4-nitrophenyl)thio]-1H-imidazole + glutathione | - |
Homo sapiens | ? | - |
? | |
4-nitrophenylacetate + glutathione | - |
Homo sapiens | ? | - |
? | |
androstenedione + glutathione | - |
Homo sapiens | ? | - |
? | |
butyl nitrite + glutathione | - |
Homo sapiens | ? | - |
? | |
cumene hydroperoxide + glutathione | - |
Homo sapiens | ? | - |
? | |
ethacrynic acid + glutathione | - |
Homo sapiens | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Homo sapiens | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
nonenal + glutathione | - |
Homo sapiens | ? | - |
? | |
phenethyl isothiocyanate + glutathione | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GST | - |
Homo sapiens |
GST A1-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |