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Literature summary for 2.5.1.18 extracted from

  • Blikstad, C.; Shokeer, A.; Kurtovic, S.; Mannervik, B.
    Emergence of a novel highly specific and catalytically efficient enzyme from a naturally promiscuous glutathione transferase (2008), Biochim. Biophys. Acta, 1780, 1458-1463.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozymes GST A1-1 and GST A4-4, expression in Escherichia coli strain XL-1 Blue, expression of mutant GIMFhelix in Escherichia coli strain Rosetta-gami B(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of mutant GIMFhelix by exchange of active site residues leading to about 300fold enhanced enzyme activity, substrate-activity profile compared to wild-type isozymes, overview. GIMFhelix contains all of the H-site residues identified in isozyme GST A4-4. GIMFhelix showed two orders of magnitude higher catalytic efficiency with long-chain alkenals than isozyme GST A1-1, while the activity with the standard substrate CDNB is one order below that of GST A1-1 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isozymes GST A1-1 and GST A4-4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozymes GST A1-1 and GST A4-4 and recombinant mutant GIMFhelix from Escherichia coli by glutathione affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyl-4-nitro-5-[(4-nitrophenyl)thio]-1H-imidazole + glutathione
-
Homo sapiens ?
-
?
4-nitrophenylacetate + glutathione
-
Homo sapiens ?
-
?
androstenedione + glutathione
-
Homo sapiens ?
-
?
butyl nitrite + glutathione
-
Homo sapiens ?
-
?
cumene hydroperoxide + glutathione
-
Homo sapiens ?
-
?
ethacrynic acid + glutathione
-
Homo sapiens ?
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Homo sapiens S-(2,4-dinitrophenyl)glutathione + HCl
-
?
nonenal + glutathione
-
Homo sapiens ?
-
?
phenethyl isothiocyanate + glutathione
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
GST
-
Homo sapiens
GST A1-1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens