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Literature summary for 2.5.1.18 extracted from

  • Chen, X.; Liu, J.; Yang, P.; Chen, D.
    Identifying functional residues in Arabidopsis thaliana zeta class glutathione S-transferase through screening inactive point mutants (2010), Biochemistry, 75, 110-114.
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
GSTZ, library screening, cloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C19Y random mutagenesis, inactive mutant Arabidopsis thaliana
K30E site-directed mutagenesis, inactive mutant Arabidopsis thaliana
K83E random mutagenesis, the mutant shows 50% reduced activity compared to the wild-type enzyme Arabidopsis thaliana
L11P site-directed mutagenesis, inactive mutant Arabidopsis thaliana
L11P/I140T site-directed mutagenesis, inactive mutant Arabidopsis thaliana
L147F random mutagenesis, the mutant shows 47.7% reduced activity compared to the wild-type enzyme Arabidopsis thaliana
L147P site-directed mutagenesis, inactive mutant Arabidopsis thaliana
L150P site-directed mutagenesis, inactive mutant Arabidopsis thaliana
L169R site-directed mutagenesis, inactive mutant Arabidopsis thaliana
L27P site-directed mutagenesis, inactive mutant Arabidopsis thaliana
L80P/N116S site-directed mutagenesis, inactive mutant Arabidopsis thaliana
additional information mutant enzyme inactivation mechanism, molecular modeling, mutations resulting in inactivation of the enzyme are found in or near the binding pocket, whereas mutations resulting in partial inactivation are distant from both substrates, overview Arabidopsis thaliana
P61S random mutagenesis, inactive mutant Arabidopsis thaliana
Q102R random mutagenesis, the mutant shows 68.4% reduced activity compared to the wild-type enzyme Arabidopsis thaliana
Q112R random mutagenesis, inactive mutant Arabidopsis thaliana
R22H/K83E random mutagenesis, inactive mutant Arabidopsis thaliana
S105R/Q213R site-directed mutagenesis, inactive mutant Arabidopsis thaliana
S109P random mutagenesis, inactive mutant Arabidopsis thaliana
S73P random mutagenesis, inactive mutant Arabidopsis thaliana
S73X site-directed mutagenesis, inactive mutant Arabidopsis thaliana
W15R random mutagenesis, inactive mutant Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dichloroacetic acid + glutathione
-
 Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
More molecular modeling of the GSTZ substrate superimposed enzyme using the apoform crystal structure of AtGSTZ monomer Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
GSTZ
-
 Arabidopsis thaliana
zeta class glutathione S-transferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Arabidopsis thaliana