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Literature summary for 2.5.1.18 extracted from

  • Wang, X.Y.; Zhang, Z.R.; Perrett, S.
    Characterization of the activity and folding of the glutathione transferase from Escherichia coli and the roles of residues Cys10 and His106 (2008), Biochem. J., 417, 55-64.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain C41 Escherichia coli

Protein Variants

Protein Variants Comment Organism
C10A shows a 5 or 6fold higher kcat than wild type for both glutathione and 1-chloro-2,4-dinitrobenzene, accompanied by an 8fold increase in the Km for glutathione Escherichia coli
C10S shows a 25fold lower kcat for glutathione H and 5fold lower kcat for 1-chloro-2,4-dinitrobenzene than wild type accompanied by a 6fold decrease in the Km for glutathione Escherichia coli
H106A shows clearly decreased activity compared to the wild type enzyme Escherichia coli
H106F shows decreased activity compared to the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
glutathione mutant enzyme C10S, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
0.3
-
glutathione wild type enzyme, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
1.3
-
1-chloro-2,4-dinitrobenzene mutant enzyme H106A, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
1.4
-
glutathione mutant enzyme H106A, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
1.9
-
glutathione mutant enzyme H106F, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
1.9
-
1-chloro-2,4-dinitrobenzene wild type enzyme, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
2.1
-
1-chloro-2,4-dinitrobenzene mutant enzyme H106F, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
2.5
-
glutathione mutant enzyme C10A, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
4.3
-
1-chloro-2,4-dinitrobenzene mutant enzyme C10A, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
4.4
-
1-chloro-2,4-dinitrobenzene mutant enzyme C10S, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A9D2 strain TG2
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity resin chromatography Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
the denaturation process is fully reversible at pH 7.5 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Escherichia coli S-(2,4-dinitrophenyl)-glutathione + HCl
-
?

Synonyms

Synonyms Comment Organism
EGST
-
Escherichia coli
glutathione S-transferase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
glutathione mutant enzyme C10S, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
1.1
-
glutathione mutant enzyme H106A, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
1.1
-
1-chloro-2,4-dinitrobenzene mutant enzyme H106A, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
1.6
-
1-chloro-2,4-dinitrobenzene mutant enzyme C10S, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
4.8
-
1-chloro-2,4-dinitrobenzene mutant enzyme H106F, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
6.8
-
glutathione mutant enzyme H106F, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
7.7
-
glutathione wild type enzyme, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
9.1
-
1-chloro-2,4-dinitrobenzene wild type enzyme, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
41
-
glutathione mutant enzyme C10A, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli
55.8
-
1-chloro-2,4-dinitrobenzene mutant enzyme C10A, at 25°C, in 100 mM phosphate buffer (pH 6.5) Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
most stable at pH 7.5 Escherichia coli