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Literature summary for 2.5.1.18 extracted from

  • Wiktelius, E.; Stenberg, G.
    Novel class of glutathione transferases from cyanobacteria exhibit high catalytic activities towards naturally occurring isothiocyanates (2007), Biochem. J., 406, 115-123.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Cibacron blue
-
Synechococcus elongatus
Cibacron blue
-
Thermosynechococcus vestitus
hexyl glutathione
-
Synechococcus elongatus
hexyl glutathione
-
Thermosynechococcus vestitus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
glutathione at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus
0.084
-
glutathione at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus
0.16
-
phenethyl-isothiocyanate at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus
0.75
-
phenethyl-isothiocyanate at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus
-
strain PCC 6301
-
Thermosynechococcus vestitus Q8DMB4
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography Synechococcus elongatus
glutathione Sepharose column chromatography Thermosynechococcus vestitus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.038
-
using H2O2 as a substrate Thermosynechococcus vestitus
0.04
-
using 4-nitrobenzyl chloride as a substrate Thermosynechococcus vestitus
0.1
-
using 4-nitrobenzylchloride as a substrate Synechococcus elongatus
0.14
-
using 1-chloro-2,4-dinitrobenzene as a substrate Thermosynechococcus vestitus
0.15
-
using ethacrynic acid as a substrate Thermosynechococcus vestitus
0.17
-
using 1-chloro-2,4-dinitrobenzene as a substrate Synechococcus elongatus
0.24
-
using cumene hydroperoxide as a substrate Thermosynechococcus vestitus
0.27
-
using ethacrynic acid as a substrate Synechococcus elongatus
0.34
-
using 1,2-epoxy-3-(p-nitrophenoxy)propane as a substrate Thermosynechococcus vestitus
0.42
-
using 1,2-epoxy-3-(p-nitrophenoxy)propane as a substrate Synechococcus elongatus
0.75
-
using cumene hydroperoxide as a substrate Synechococcus elongatus
1.54
-
using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as a substrate Thermosynechococcus vestitus
5
-
using sulforaphane as a substrate Thermosynechococcus vestitus
5.26
-
using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as a substrate Synechococcus elongatus
6
-
using propyl-isothiocyanate as a substrate Synechococcus elongatus
8
-
using propyl-isothiocyanate as a substrate Thermosynechococcus vestitus
10.04
-
using trans-2-nonenal as a substrate Thermosynechococcus vestitus
11
-
using cyclohexyl-isothiocyanate as a substrate Thermosynechococcus vestitus
14
-
using allyl-isothiocyanate as a substrate Thermosynechococcus vestitus
15
-
using allyl-isothiocyanate as a substrate Synechococcus elongatus
21
-
using cyclohexyl-isothiocyanate as a substrate Synechococcus elongatus
26
-
using sulforaphane as a substrate Synechococcus elongatus
33
-
using phenethyl-isothiocyanate as a substrate Thermosynechococcus vestitus
102
-
using phenethyl-isothiocyanate as a substrate Synechococcus elongatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1,2-epoxy-3-(p-nitrophenoxy)propane
-
Synechococcus elongatus ?
-
?
glutathione + 1,2-epoxy-3-(p-nitrophenoxy)propane
-
Thermosynechococcus vestitus ?
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Synechococcus elongatus S-(2,4-dinitrophenyl)-glutathione + HCl
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Thermosynechococcus vestitus S-(2,4-dinitrophenyl)-glutathione + HCl
-
?
glutathione + 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole
-
Synechococcus elongatus ?
-
?
glutathione + 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole
-
Thermosynechococcus vestitus ?
-
?
glutathione + 4-nitrobenzyl chloride
-
Thermosynechococcus vestitus ?
-
?
glutathione + 4-nitrobenzylchloride
-
Synechococcus elongatus ?
-
?
glutathione + allyl-isothiocyanate
-
Synechococcus elongatus ?
-
?
glutathione + allyl-isothiocyanate
-
Thermosynechococcus vestitus ?
-
?
glutathione + cumene hydroperoxide
-
Synechococcus elongatus ?
-
?
glutathione + cumene hydroperoxide
-
Thermosynechococcus vestitus ?
-
?
glutathione + cyclohexyl-isothiocyanate
-
Synechococcus elongatus ?
-
?
glutathione + cyclohexyl-isothiocyanate
-
Thermosynechococcus vestitus ?
-
?
glutathione + ethacrynic acid
-
Synechococcus elongatus ?
-
?
glutathione + ethacrynic acid
-
Thermosynechococcus vestitus ?
-
?
glutathione + H2O2
-
Thermosynechococcus vestitus ?
-
?
glutathione + phenethyl-isothiocyanate
-
Synechococcus elongatus ?
-
?
glutathione + phenethyl-isothiocyanate
-
Thermosynechococcus vestitus ?
-
?
glutathione + propyl-isothiocyanate
-
Synechococcus elongatus ?
-
?
glutathione + propyl-isothiocyanate
-
Thermosynechococcus vestitus ?
-
?
glutathione + sulforaphane
-
Synechococcus elongatus ?
-
?
glutathione + sulforaphane
-
Thermosynechococcus vestitus ?
-
?
glutathione + trans-2-nonenal
-
Thermosynechococcus vestitus ?
-
?
additional information no activity with 1,2-dichloro-4-nitrobenzene, iodomethane, 1-iodopenthane, and dichloromethane Thermosynechococcus vestitus ?
-
?
additional information no activity with 1,2-dichloro-4-nitrobenzene, iodomethane, 1-iodopenthane, H2O2, trans-2-nonenal, and dichloromethane Synechococcus elongatus ?
-
?

Synonyms

Synonyms Comment Organism
Chi class GST
-
Synechococcus elongatus
Chi class GST
-
Thermosynechococcus vestitus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
almost 100% of its activity retained after incubation at 70°C Thermosynechococcus vestitus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
glutathione at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus
21.4
-
phenethyl-isothiocyanate at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus
48.7
-
glutathione at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus
63.4
-
phenethyl-isothiocyanate at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00000075
-
at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus Cibacron blue
0.00005
-
at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus Cibacron blue
0.15
-
at 30°C in 0.1 M potassium phosphate buffer Synechococcus elongatus hexyl glutathione
0.5
-
at 30°C in 0.1 M potassium phosphate buffer Thermosynechococcus vestitus hexyl glutathione