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Literature summary for 2.5.1.18 extracted from
- 2-Hydroxychromene-2-carboxylic acid isomerase: a kappa class glutathione transferase from Pseudomonas putida (2007), Biochemistry, 46, 6710-6722.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified enzyme complexed with glutathione, 2-hydroxychromene-2-carboxylic acid, and trans-o-hydroxybenzylidene pyruvic acid, 10 mg/ml protein in storage buffer, with 1 mM GSH, and 1 mM HCCA at 21°C, all crystals are grown under 7 ml of Als oil in a microbatch plate, X-ray diffraction structure determination and analysis at 1.7 A resolution, structure modeling | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| trans-o-hydroxybenzylidene pyruvic acid | - |
Pseudomonas putida |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state and ligand binding kinetics, recombinant enzyme | Pseudomonas putida | |
| 0.017 | - |
reduced glutathione | pH 7.0, 25°C, recombinant enzyme | Pseudomonas putida | |
| 0.084 | - |
2-hydroxychromene-2-carboxylic acid | pH 7.0, 25°C, recombinant enzyme | Pseudomonas putida | |
| 0.138 | - |
trans-o-hydroxybenzylidene pyruvic acid | pH 7.0, 25°C, recombinant enzyme | Pseudomonas putida | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Pseudomonas putida | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxychromene-2-carboxylic acid | Pseudomonas putida | HCCA isomerase catalyzes the glutathione-dependent interconversion of 2-hydroxychromene-2-carboxylic acid and trans-o-hydroxybenzylidene pyruvic acid in the naphthalene catabolic pathway of Pseudomonas putida, mechanisms in the naphthalene catabolic pathwayoverview | trans-o-hydroxybenzylidene pyruvic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble enzyme from supernatant by anion exchange chromatograpy and gel filtration | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 270 | - |
purified recombinant enzyme in presence of reduced glutathione | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxychromene-2-carboxylic acid | HCCA isomerase catalyzes the glutathione-dependent interconversion of 2-hydroxychromene-2-carboxylic acid and trans-o-hydroxybenzylidene pyruvic acid in the naphthalene catabolic pathway of Pseudomonas putida, mechanisms in the naphthalene catabolic pathwayoverview | Pseudomonas putida | trans-o-hydroxybenzylidene pyruvic acid | - |
? | |
| 2-hydroxychromene-2-carboxylic acid | the dimeric protein binds one molecule of GSH very tightly and a second molecule of GSH with much lower affinity, GSH acts as a tightly bound cofactor in the reaction, binding structure, overview | Pseudomonas putida | trans-o-hydroxybenzylidene pyruvic acid | - |
? | |
| 2-hydroxychromene-2-carboxylic acid + reduced glutathione | - |
Pseudomonas putida | trans-o-hydroxybenzylidene pyruvic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-hydroxychromene-2-carboxylic acid isomerase | - |
Pseudomonas putida |
| HCCA isomerase | - |
Pseudomonas putida |
| kappa class glutathione transferase | - |
Pseudomonas putida |
| kappa class GSH transferase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19 | - |
trans-o-hydroxybenzylidene pyruvic acid | pH 7.0, 25°C, recombinant enzyme | Pseudomonas putida | |
| 39 | - |
reduced glutathione | pH 7.0, 25°C, recombinant enzyme | Pseudomonas putida | |
| 47 | - |
2-hydroxychromene-2-carboxylic acid | pH 7.0, 25°C, recombinant enzyme | Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| GSH | binding structure, overview | Pseudomonas putida | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.136 | - |
trans-o-hydroxybenzylidene pyruvic acid | pH 7.0, 25°C, recombinant enzyme | Pseudomonas putida | |
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