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Literature summary for 2.5.1.18 extracted from

  • Hamed, R.R.; Abu-Shady, M.R.; El-Beih, F.M.; Abdalla, A.M.; Afifi, O.M.
    Purification and characterization of a glutathione S-transferase from Mucor mucedo (2005), Pol. J. Microbiol., 54, 153-160.
    View publication on PubMed
Search for more literature for 2.5.1.18

Inhibitors

Inhibitors Comment Organism Structure
Cibacron blue
-
 Mucor mucedo
Hematin
-
 Mucor mucedo

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the enzyme does not show Michaelis-Menten kinetics Mucor mucedo
0.179
-
 glutathione pH 8.0, substrate concentration 0.1-0.3 mM Mucor mucedo
0.531
-
 1-chloro-2,4-dinitrobenzene pH 8.0, substrate concentration 0.1-0.3 mM Mucor mucedo
0.65
-
 glutathione pH 8.0, substrate concentration 0.3-2.0 mM Mucor mucedo
11
-
 1-chloro-2,4-dinitrobenzene pH 8.0, substrate concentration 0.3-2.0 mM Mucor mucedo

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23400
-
 2 * 23400, SDS-PAGE Mucor mucedo
53000
-
 gel filtration Mucor mucedo

Organism

Organism UniProt Comment Textmining
Mucor mucedo
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange and glutathione affinity chromatography Mucor mucedo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Mucor mucedo chloride + 2,4-dinitrophenyl-glutathione
-
 ?
additional information the enzyme shows nearly no glutathione peroxidase activity Mucor mucedo ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23400, SDS-PAGE Mucor mucedo

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
 Mucor mucedo
GST
-
 Mucor mucedo

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
39.8
-
 glutathione pH 8.0, substrate concentration 0.1-0.3 mM Mucor mucedo
39.8
-
 1-chloro-2,4-dinitrobenzene pH 8.0, substrate concentration 0.1-0.3 mM Mucor mucedo
552
-
 glutathione pH 8.0, substrate concentration 0.3-2.0 mM Mucor mucedo
552
-
 1-chloro-2,4-dinitrobenzene pH 8.0, substrate concentration 0.3-2.0 mM Mucor mucedo

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Mucor mucedo

pI Value

Organism Comment pI Value Maximum pI Value
Mucor mucedo
-
-
 4.8

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000252
-
 pH 8.0 Mucor mucedo Cibacron blue
0.00355
-
 pH 8.0 Mucor mucedo Hematin