Protein Variants | Comment | Organism |
---|---|---|
F35L | site-directed mutagenesis, the mutant shows highly reduced activity with alachlor compared to the wild-type enzyme | Zea mays |
I118F | site-directed mutagenesis, the mutant shows increased activity with alachlor compared to the wild-type enzyme | Zea mays |
W12I | site-directed mutagenesis, the mutant shows reduced activity with alachlor compared to the wild-type enzyme | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics and kinetics of recombinant wild-type and mutant enzymes, the rate-limiting step of the reaction is viscosity-dependent product release | Zea mays | |
0.042 | - |
alachlor | pH 6.5, 30°C, recombinant mutant I118F | Zea mays | |
0.18 | - |
alachlor | pH 6.5, 30°C, recombinant wild-type enzyme | Zea mays | |
0.73 | - |
glutathione | pH 6.5, 30°C, recombinant mutant W12I | Zea mays | |
0.76 | - |
glutathione | pH 6.5, 30°C, recombinant mutant I118F | Zea mays | |
0.82 | - |
alachlor | pH 6.5, 30°C, recombinant mutant W12I | Zea mays | |
0.83 | - |
glutathione | pH 6.5, 30°C, recombinant wild-type enzyme | Zea mays | |
3.92 | - |
glutathione | pH 6.5, 30°C, recombinant mutant F35L | Zea mays | |
7.23 | - |
alachlor | pH 6.5, 30°C, recombinant mutant F35L | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + alachlor | Zea mays | isozyme GSTI | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
isozyme GST I | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
RX + glutathione = HX + R-S-glutathione | the enzyme uses a rapid equilibrium random sequential bi-bi mechanism with intrasubunit modulation between GSH binding site, the G-site, and electrophile binding site, the H-site, Trp12, Phe35 and Ile118 are involved in substrate binding, Phe35 is important for catalytic activity | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Zea mays | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
glutathione + alachlor | isozyme GSTI | Zea mays | ? | - |
? | |
glutathione + alachlor | i.e. 2-chloro-N-[2,6-diethylphenyl]-N-[methoxymethyl]acetamide, isozyme GSTI | Zea mays | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Zea mays |
GST | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
alachlor | pH 6.5, 30°C, recombinant mutant W12I | Zea mays | |
0.00019 | - |
alachlor | pH 6.5, 30°C, recombinant mutant F35L | Zea mays | |
0.00046 | - |
alachlor | pH 6.5, 30°C, recombinant mutant I118F | Zea mays | |
0.00048 | - |
alachlor | pH 6.5, 30°C, recombinant wild-type enzyme | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Zea mays |