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Literature summary for 2.5.1.18 extracted from
- Biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm Ancylostoma caninum (2005), Infect. Immun., 73, 6903-6911.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | isozyme GST-1 is a vaccine target | Ancylostoma caninum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gst-1, DNA and amino acid sequence determination and analysis, expression of His-tagged GST-1 in Pichia pastoris strain X-33 | Ancylostoma caninum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 24000, isozyme GST-1, SDS-PAGE | Ancylostoma caninum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + hematin | Ancylostoma caninum | GST-1 is involved in parasite heme detoxification during hemoglobin digestion or heme uptake | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ancylostoma caninum | Q6J1M5 | GST-1; canine hookworm, gene gst-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged GST-1 from Pichia pastoris strain X-33 by nickel affinity chromatography | Ancylostoma caninum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adult | isozyme GST-1 | Ancylostoma caninum | - |
| hypodermis | - |
Ancylostoma caninum | - |
| intestine | low activity | Ancylostoma caninum | - |
| additional information | developmental expression profile, mRNA encoding GST-1 is detected in adults, eggs, and larval stages, but the protein is detected only in adult hookworm somatic extracts and excretory/secretory products, overview | Ancylostoma caninum | - |
| muscle | - |
Ancylostoma caninum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Ancylostoma caninum | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
| glutathione + hematin | GST-1 is involved in parasite heme detoxification during hemoglobin digestion or heme uptake | Ancylostoma caninum | ? | - |
? | |
| glutathione + hematin | high affinity binding by GST-1 | Ancylostoma caninum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000, isozyme GST-1, SDS-PAGE | Ancylostoma caninum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Ancylostoma caninum |
| GST-1 | - |
Ancylostoma caninum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Ancylostoma caninum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Ancylostoma caninum |
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