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Literature summary for 2.5.1.18 extracted from
- Glutathione S-transferase from the Icelandic scallop (Chlamys islandica): isolation and partial characterization (2007), Comp. Biochem. Physiol. C, 144C, 403-407.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression analysis | Chlamys islandica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
glutathione | pH 6.5, 24°C | Chlamys islandica |  |
| 0.4 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 24°C | Chlamys islandica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
2 * 22000, SDS-PAGE | Chlamys islandica |
| 44000 | - |
gel filtration | Chlamys islandica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamys islandica | Q0P7I5 | a cold-adapted marine bivalve Iceandic scallop, mu-class enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by glutathione affinity chromatography to homogeneity | Chlamys islandica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive gland | enzyme expression | Chlamys islandica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 300.6 | - |
purified native enzyme | Chlamys islandica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Chlamys islandica | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 22000, SDS-PAGE | Chlamys islandica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Chlamys islandica |
| GST | - |
Chlamys islandica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
assay at | Chlamys islandica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
55 | maximal activity at 0°C, 10% of maximal activity at 55%, inactive at 60°C | Chlamys islandica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
10 min, loss of 20% of activity | Chlamys islandica |
| 60 | - |
purified enzyme, rapid complete inactivation | Chlamys islandica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Chlamys islandica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | no activity at pH 4.5, 10% of maximal activity at pH 5.0, 60% at pH 8.0 | Chlamys islandica |
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Release 2023.1 (January 2023)
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