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Literature summary for 2.5.1.18 extracted from
- Inhibition of cytosolic glutathione S-transferase activity from rat liver by copper (2006), Chem. Biol. Interact., 164, 39-48.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | ascorbate enhances the inhibitory effects of SH reagents, overview, inactivation of total cytosolic GST activity from liver by the oxygen radical-generating system Cu2+/ascorbate, two mechanisms: ROS-induced oxidation and non-specific Cu2+ binding to protein thiol groups | Rattus norvegicus |  |
| Cu2+ | inhibitory in either the absence or presence of ascorbate, inactivation of total cytosolic GST activity from liver by the oxygen radical-generating system Cu2+/ascorbate, two mechanisms: ROS-induced oxidation and non-specific Cu2+ binding to protein thiol groups, the inhibition is prevented by glutathione but not 1-chloro-2,4-dinitrobenzene, Cu2+ inhibition affects the Km for glutathione but not of 1-chloro-2,4-dinitrobenzene, overview | Rattus norvegicus | |
| H2O2 | inactivation of cytosolic GST activity from liver | Rattus norvegicus | |
| Mersalyl acid | - |
Rattus norvegicus | |
| NEM | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Cu2+ effects on Km, overview | Rattus norvegicus | |
| 0.11 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 25°C | Rattus norvegicus | |
| 0.19 | - |
glutathione | pH 6.5, 25°C | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially by subcellular fractionation | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Rattus norvegicus | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Rattus norvegicus |
| GST | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0045 | - |
Mersalyl acid | pH 6.5, 25°C | Rattus norvegicus | |
| 0.2 | - |
NEM | pH 6.5, 25°C | Rattus norvegicus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus | additional information | |
| 0.0051 | - |
pH 6.5, 25°C | Rattus norvegicus | Cu2+ | |
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