Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.18 extracted from

  • Rufer, A.C.; Thiebach, L.; Baer, K.; Klein, H.W.; Hennig, M.
    X-ray structure of glutathione S-transferase from Schistosoma japonicum in a new crystal form reveals flexibility of the substrate-binding site (2005), Acta Crystallogr. Sect. F, 61, 263-265.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.18

Application

Application Comment Organism
drug development the enzyme is a target for inhibitor design Schistosoma japonicum

Cloned(Commentary)

Cloned (Comment) Organism
constitutive gst gene, expression of the enzyme as fusion protein with the full-length intracellular domain of the human insulin receptor, i.e. GST-IRK, comprising the coding region of IRK, residues Val966–Lys1271, C969S, Y972F, and containing a thrombin-cleavage site as a linker between GSTand IRK, in Spodoptera frugiperda Sf9 cells Schistosoma japonicum

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant GST-IRK, 10 mg/ml protein in 20 mM Tris–HCl, pH 7.5, 150 mM NaCl, and 2 mM TCEP, 21°C, against 0.1 M Bis-Tris, pH 5.5, and 25% w/v PEG 3350, or 0.1 M sodium acetate pH 5.5, and 36% w/v PEG MME 5000 as precipitant solutions, 7 days, orthorhombic crystals, X-ray diffraction structure determination and analysis, at 3.0-3.14 A resolution, molecular-replacement Schistosoma japonicum

Inhibitors

Inhibitors Comment Organism Structure
praziquantel
-
 Schistosoma japonicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Schistosoma japonicum the enzyme is the major detoxification enzyme of Schistosoma japonicum, a pathogenic helminth causing schistosomiasis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Schistosoma japonicum P08515
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is the major detoxification enzyme of Schistosoma japonicum, a pathogenic helminth causing schistosomiasis Schistosoma japonicum ?
-
 ?

Subunits

Subunits Comment Organism
More structure analysis shows flexibility of the substrate-binding site Schistosoma japonicum

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
 Schistosoma japonicum
GST
-
 Schistosoma japonicum