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Literature summary for 2.5.1.18 extracted from

  • Papadopoulos, A.I.; Polemitou, I.; Laifi, P.; Yiangou, A.; Tananaki, C.
    Glutathione S-transferase in the insect Apis mellifera macedonica kinetic characteristics and effect of stress on the expression of GST isoenzymes in the adult worker bee (2004), Comp. Biochem. Physiol. C, 139, 93-97.
    View publication on PubMed
Search for more literature for 2.5.1.18

Inhibitors

Inhibitors Comment Organism Structure
bromosulfophthalein 0.053 mM, 50% inhibition, isoenzyme I; 1.24 mM, 50% inhibition, isoenzyme II Apis mellifera macedonica
chlorotriphenyltin 0.33 mM, 50% inhibition, isoenzyme I; 6.4 mM, 50% inhibition, isoenzyme II Apis mellifera macedonica
Cibacron blue 0.053 mM, 50% inhibition, isoenzyme I; 0.073 mM, 50% inhibition, isoenzyme II Apis mellifera macedonica
Hematin 0.13 mM, 50% inhibition, isoenzyme I; 1.81 mM, 50% inhibition, isoenzyme II Apis mellifera macedonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00026
-
 1-chloro-2,4-dinitrobenzene isoenzyme I Apis mellifera macedonica
0.00027
-
 GSH isoenzyme I Apis mellifera macedonica
0.00089
-
 1-chloro-2,4-dinitrobenzene isoenzyme I Apis mellifera macedonica
0.0012
-
 GSH isoenzyme I Apis mellifera macedonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 1 * 29000 + 1 * 25000, SDS-PAGE Apis mellifera macedonica
29000
-
 1 * 29000 + 1 * 25000, SDS-PAGE Apis mellifera macedonica

Organism

Organism UniProt Comment Textmining
Apis mellifera macedonica
-
 isoenzyme I
-
Apis mellifera macedonica
-
 isoenzyme II
-

Purification (Commentary)

Purification (Comment) Organism
-
 Apis mellifera macedonica

Source Tissue

Source Tissue Comment Organism Textmining
whole body homogenate of adult worker bee Apis mellifera macedonica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + GSH
-
 Apis mellifera macedonica ?
-
 ?
1-chloro-2,4-dinitrobenzene + GSH
-
 Apis mellifera macedonica S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
bromosulfophthalein + GSH 10.3% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme II Apis mellifera macedonica ?
-
 ?
bromosulfophthalein + GSH 8.6% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme I Apis mellifera macedonica ?
-
 ?
cumene hydroperoxide + GSH 106% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme I Apis mellifera macedonica ?
-
 ?
cumene hydroperoxide + GSH 11.9% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme II Apis mellifera macedonica ?
-
 ?
ethacrynic acid + GSH 15% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme I Apis mellifera macedonica ?
-
 ?
GSH reductase + GSH 271% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme II Apis mellifera macedonica ?
-
 ?
GSH reductase + GSH 87% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme I Apis mellifera macedonica ?
-
 ?
trans-phenyl-butenone + GSH 312% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme II Apis mellifera macedonica ?
-
 ?
trans-phenyl-butenone + GSH 71% of the activity with 1-chloro-2,4-dinitrobenzene, isoenzyme I Apis mellifera macedonica ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 29000 + 1 * 25000, SDS-PAGE Apis mellifera macedonica