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Literature summary for 2.5.1.18 extracted from

  • Park, H.J.; Koh, J.U.; Ahn, S.Y.; Kong, K.H.
    Functional studies of tyrosine 108 residue in the active site of human glutathione S-transferase P1-1 (2005), Bull. Korean Chem. Soc., 26, 433-439.
No PubMed abstract available
Search for more literature for 2.5.1.18

Protein Variants

Protein Variants Comment Organism
Y108A mutant enzyme shows 58% of the wild-type activity with 1,2-dichloro-4-nitrobenzene as substrate and 37% of the wild-type activity with 1,2-epoxy-3-(p-nitrophenoxy)propane, 2% of the wild-type GSH peroxidase activity towards cumene hydroperoxide and 163% of the wild-type steroid isomerase activity towards DELTA5-androstene-3,17-dione Homo sapiens
Y108F mutant enzyme is as active as wild-type enzyme with 1,2-dichloro-4-nitrobenzene as substrate and shows 7% of the wild-type activity with 1,2-epoxy-3-(p-nitrophenoxy)propane, 77% of the wild-type GSH peroxidase activity towards cumene hydroperoxide and 149% of the wild-type steroid isomerase activity towards DELTA5-androstene-3,17-dione. Mutation results in an approximately 3fold decrease in the I50 value of S-methyl-GSH Homo sapiens
Y108W mutant enzyme shows 215% of the wild-type activity with 1,2-dichloro-4-nitrobenzene as substrate and 35% of the wild-type activity with 1,2-epoxy-3-(p-nitrophenoxy)propane, 16% of the wild-type GSH peroxidase activity towards cumene hydroperoxide and 36% of the wild-type steroid isomerase activity towards DELTA5-androstene-3,17-dione. Mutation results in an approximately 3fold decrease in the I50 value of S-methyl-GSH. Lower heat stability than wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Hematin
-
 Homo sapiens
S-(2,4-dinitrophenyl)GSH
-
 Homo sapiens
S-methyl-GSH
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 GSH 30°C, mutant enzyme Y108F, reaction with 1,2-dichloro-4-nitrobenzene Homo sapiens
0.09
-
 GSH 30°C, mutant enzyme Y108W, reaction with 1,2-dichloro-4-nitrobenzene Homo sapiens
0.13
-
 1,2-epoxy-3-(p-nitrophenoxy)propane 30°C, mutant enzyme Y108W Homo sapiens
0.16
-
 GSH 30°C, mutant enzyme Y108F, reaction with cumene hydroperoxide Homo sapiens
0.17
-
 GSH 30°C, wild-type enzyme, reaction with cumene hydroperoxide Homo sapiens
0.2
-
 GSH 30°C, mutant enzyme Y108W, reaction with cumene hydroperoxide Homo sapiens
0.25
-
 1,2-epoxy-3-(p-nitrophenoxy)propane 30°C, wild-type enzyme Homo sapiens
0.27
-
 1,2-dichloro-4-nitrobenzene 30°C, mutant enzyme Y108W Homo sapiens
0.29
-
 GSH 30°C, wild-type enzyme, reaction with 1,2-dichloro-4-nitrobenzene Homo sapiens
0.3
-
 cumene hydroperoxide 30°C, wild-type enzyme Homo sapiens
0.4
-
 GSH 30°C, mutant enzyme Y108A, reaction with 1,2-dichloro-4-nitrobenzene Homo sapiens
0.4
-
 GSH 30°C, mutant enzyme Y108A, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane Homo sapiens
0.4
-
 GSH 30°C, wild-type enzyme, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane Homo sapiens
0.45
-
 1,2-dichloro-4-nitrobenzene 30°C, mutant enzyme Y108F Homo sapiens
0.46
-
 GSH 30°C, mutant enzyme Y108F, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane Homo sapiens
0.48
-
 GSH 30°C, mutant enzyme Y108W, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane Homo sapiens
0.65
-
 1,2-dichloro-4-nitrobenzene 30°C, wild-type enzyme Homo sapiens
0.84
-
 1,2-dichloro-4-nitrobenzene 30°C, mutant enzyme Y108A Homo sapiens
0.88
-
 GSH 30°C, pH 6.5, wild-type enzyme, reaction with DELTA5-androstene-3,17-dione Homo sapiens
0.9
-
 cumene hydroperoxide 30°C, mutant enzyme Y108W Homo sapiens
1.3
-
 1,2-epoxy-3-(p-nitrophenoxy)propane 30°C, mutant enzyme Y108A Homo sapiens
1.76
-
 GSH 30°C, mutant enzyme Y108A, reaction with cumene hydroperoxide Homo sapiens
1.84
-
 1,2-epoxy-3-(p-nitrophenoxy)propane 30°C, mutant enzyme Y108F Homo sapiens
2.11
-
 cumene hydroperoxide 30°C, mutant enzyme Y108F Homo sapiens
8
-
 cumene hydroperoxide 30°C, mutant enzyme Y108A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 enzyme form glutathione S-transferase P1-1, recombinant
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dichloro-4-nitrobenzene + GSH
-
 Homo sapiens ?
-
 ?
1,2-epoxy-3-(p-nitrophenoxy)propane + GSH
-
 Homo sapiens ?
-
 ?
cumene hydroperoxide + GSH
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
glutathione S-transferase P1-1
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 7, 10 min, wild-type enzyme and mutant enzymes Y108A and Y108F are stable up to, inactivation above Homo sapiens
53
-
 midpoint of inactivation of mutant enzyme Y108W Homo sapiens
77
-
 midpoint of inactivation of wild-type enzyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31
-
 1,2-epoxy-3-(p-nitrophenoxy)propane 30°C, mutant enzyme Y108F Homo sapiens
107
-
 GSH 30°C, mutant enzyme Y108F, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane Homo sapiens
108
-
 GSH 30°C, wild-type enzyme, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane Homo sapiens
110
-
 1,2-dichloro-4-nitrobenzene 30°C, mutant enzyme Y108A Homo sapiens
110
-
 1,2-dichloro-4-nitrobenzene 30°C, mutant enzyme Y108F Homo sapiens
110
-
 GSH 30°C, mutant enzyme Y108F, reaction with 1,2-dichloro-4-nitrobenzene Homo sapiens
112
-
 1,2-epoxy-3-(p-nitrophenoxy)propane 30°C, mutant enzyme Y108A Homo sapiens
132
-
 GSH 30°C, mutant enzyme Y108A, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane Homo sapiens
150
-
 GSH 30°C, mutant enzyme Y108W, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane Homo sapiens
200
-
 cumene hydroperoxide 30°C, mutant enzyme Y108A Homo sapiens
201
-
 1,2-epoxy-3-(p-nitrophenoxy)propane 30°C, wild-type enzyme Homo sapiens
210
-
 1,2-dichloro-4-nitrobenzene 30°C, wild-type enzyme Homo sapiens
302
-
 1,2-epoxy-3-(p-nitrophenoxy)propane 30°C, mutant enzyme Y108W Homo sapiens
320
-
 1,2-dichloro-4-nitrobenzene 30°C, mutant enzyme Y108W Homo sapiens
410
-
 GSH 30°C, mutant enzyme Y108A, reaction with 1,2-dichloro-4-nitrobenzene Homo sapiens
410
-
 GSH 30°C, wild-type enzyme, reaction with 1,2-dichloro-4-nitrobenzene Homo sapiens
700
-
 GSH 30°C, mutant enzyme Y108W, reaction with 1,2-dichloro-4-nitrobenzene Homo sapiens
1020
-
 GSH 30°C, mutant enzyme Y108F, reaction with cumene hydroperoxide Homo sapiens
1370
-
 GSH 30°C, wild-type enzyme, reaction with cumene hydroperoxide Homo sapiens
1610
-
 GSH 30°C, mutant enzyme Y108W, reaction with cumene hydroperoxide Homo sapiens
1700
-
 GSH 30°C, mutant enzyme Y108A, reaction with cumene hydroperoxide Homo sapiens
2700
-
 cumene hydroperoxide 30°C, mutant enzyme Y108W Homo sapiens
8100
-
 cumene hydroperoxide 30°C, mutant enzyme Y108F Homo sapiens
9600
-
 cumene hydroperoxide 30°C, wild-type enzyme Homo sapiens