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Literature summary for 2.5.1.18 extracted from

  • Board, P.G.; Taylor, M.C.; Coggan, M.; Parker, M.W.; Lantum, H.B.; Anders, M.W.
    Clarification of the role of key active site residues of glutathione transferase zeta/maleylacetoacetate isomerase by a new spectrophotometric technique (2003), Biochem. J., 374, 731-737.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
C16A mutation causes a high increase in the KM-value. Mutation diminishes activity with both chlorofluoroacetic acid and maleylacetone but shows significantly elevated activity with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid as a substrate. This elevated activity appears to be driven by a high turnover number. The diminished activity with maleylacetone results from a low turnover number Homo sapiens
R175A mutation results in a significant lowering of the turnover number for (+)-2-bromo-3-(4-nitrophenyl)propanoic acid and maleylacetone, elevated KM-value for maleylacetone Homo sapiens
R175K mutation results in a significant lowering of the turnover number for (+)-2-bromo-3-(4-nitrophenyl)propanoic acid and maleylacetone Homo sapiens
S14A inactive mutant enzyme Homo sapiens
S15A mutant enzyme shows low isomerase activity with maleylacetone as a substrate (about 15% of wild-type) and low activity with chlorofluoroacetic acid, it shows elevated activity with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid as the substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0139
-
GSH pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
0.0146
-
GSH pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
0.0178
-
GSH pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
0.023
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c Homo sapiens
0.025
-
GSH pH 7.4, 37°C, glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
0.027
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c Homo sapiens
0.029
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1d-1d Homo sapiens
0.044
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1c-1c Homo sapiens
0.047
-
GSH pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
0.064
-
GSH pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
0.064
-
maleylacetone pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c Homo sapiens
0.068
-
maleylacetone pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c Homo sapiens
0.096
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1b-1b Homo sapiens
0.117
-
GSH pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
0.128
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1a-1a Homo sapiens
0.134
-
GSH pH 7.4, 37°C, glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
0.223
-
maleylacetone pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c Homo sapiens
0.287
-
GSH pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
0.298
-
GSH pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
0.319
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c Homo sapiens
0.347
-
maleylacetone pH 7.4, 37°C, glutathione transferase Zeta 1c-1c Homo sapiens
0.417
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c Homo sapiens
0.768
-
maleylacetone pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme plays an important role in the metabolism of tyrosine via its maleylacetoacetate isomerase activity ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
spectrophotometric assay with 2-bromo-3-(4-nitrophenyl)propionic acid as substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid + glutathione
-
Homo sapiens 2-(glutathion-S-yl)-3-(4-nitrophenyl)propanoic acid + HBr
-
?
chlorofluoroacetic acid + glutathione
-
Homo sapiens ?
-
?
dichloroacetic acid + glutathione
-
Homo sapiens ?
-
?
maleylacetone + glutathione
-
Homo sapiens ?
-
?
additional information the enzyme plays an important role in the metabolism of tyrosine via its maleylacetoacetate isomerase activity Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
glutathione transferase zeta
-
Homo sapiens
glutathione transferase Zeta 1-1 variants glutathione transferase Zeta 1a-1a, glutathione transferase Zeta 1b-1b, glutathione transferase Zeta 1c-1c, glutathione transferase Zeta 1d-1d Homo sapiens
hGSTZ1-1
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c Homo sapiens
0.19
-
GSH pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
0.347
-
maleylacetone pH 7.4, 37°C, glutathione transferase Zeta 1c-1c Homo sapiens
0.55
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1d-1d Homo sapiens
0.59
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1c-1c Homo sapiens
0.61
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c Homo sapiens
0.61
-
GSH pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
0.82
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1b-1b Homo sapiens
1
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c Homo sapiens
1
-
GSH pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
2.55
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c Homo sapiens
2.55
-
GSH pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens
13
-
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1a-1a Homo sapiens
72.6
-
maleylacetone pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c Homo sapiens
72.6
-
GSH pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
141
-
maleylacetone pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c Homo sapiens
141
-
GSH pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
149
-
maleylacetone pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c Homo sapiens
149
-
GSH pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
368
-
maleylacetone pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c Homo sapiens
368
-
GSH pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens
464
-
maleylacetone pH 7.4, 37°C, glutathione transferase Zeta 1c-1c Homo sapiens
464
-
GSH pH 7.4, 37°C, glutathione transferase Zeta 1c-1c, reaction with maleylacetone Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
activity with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 11 pH 6.5: about 65% of maximal activity, pH 11: about 85% of maximal activity, activity with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid Homo sapiens