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Literature summary for 2.5.1.18 extracted from

  • Asaoka, K.; Takahashi, K.
    Monkey glutathione S-aryltransferases. II. Properties of the major enzyme purified from the liver (1977), J. Biochem., 82, 1313-1323.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzenesulfonate
-
Macaca mulatta
benzene hexachloride
-
Macaca mulatta
Cd2+ 3.3 mM Macaca mulatta
Co2+ 3.3 mM Macaca mulatta
Cu2+ 3.3 mM Macaca mulatta
guanidine hydrochloride reversible denaturation at 3.2 M Macaca mulatta
Hg2+ 3.3 mM Macaca mulatta
iodoacetate weak Macaca mulatta
N-ethylmaleimide weak Macaca mulatta
Ni2+ 3.3 mM Macaca mulatta
Phenylglyoxal
-
Macaca mulatta
Sodium dodecyl sulfate denaturates irreversibly at 0.03 M Macaca mulatta
Urea denaturates at 7.2 M, reversible Macaca mulatta
vinylpyridine weak Macaca mulatta
Zn2+ 3.3 mM Macaca mulatta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
glutathione
-
Macaca mulatta
1.4
-
1,2-dichloro-4-nitrobenzene
-
Macaca mulatta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
2 * 24000, SDS-PAGE Macaca mulatta
48000
-
isoenzyme Macaca mulatta

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone stable for 2 h at 5% and 25% acetone Macaca mulatta
Butanol 90% inactivation at 25% butanol, stable for 2 h at 5% butanol Macaca mulatta
dimethyl sulfoxide 5% and 25% dimethyl sulfoxide: stable for 2 h Macaca mulatta
dimethylformamide stable for 2 h at 5% and 25% dimethylformamide Macaca mulatta
dioxane 5% dioxane: stable for 2 h Macaca mulatta
dioxane 25% dioxane: 25% loss of activity Macaca mulatta
Ethanol 5% and 25% ethanol: stable for 2 h Macaca mulatta
Methanol stable at 5% and 25% methanol for 2 h Macaca mulatta
methyl cellosolve stable at 5% and 25% methyl cellosolve for 2 h Macaca mulatta
propanol stable for 2 h at 5% propanol Macaca mulatta
propanol 90% loss of activity at 25% propanol Macaca mulatta

Organism

Organism UniProt Comment Textmining
Macaca mulatta
-
rhesus monkey
-

Purification (Commentary)

Purification (Comment) Organism
-
Macaca mulatta

Renatured (Commentary)

Renatured (Comment) Organism
partial regeneration after urea and guanidine hydrochloride denaturation Macaca mulatta

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Macaca mulatta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1,2-dichloro-4-nitrobenzene
-
Macaca mulatta ?
-
?
glutathione + 4-nitrobenzyl chloride
-
Macaca mulatta ?
-
?
glutathione + 4-nitrophenyl bromide
-
Macaca mulatta ?
-
?
glutathione + trans-4-phenyl-3-buten-2-one
-
Macaca mulatta ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 24000, SDS-PAGE Macaca mulatta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 7.5, 3 h, stable Macaca mulatta
40
-
10 min, pH 7.5, stable up to 40°C Macaca mulatta
40
-
moderately stable below Macaca mulatta
50
-
complete inactivation within 1 h Macaca mulatta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9 1,2-dichloro-4-nitrobenzene Macaca mulatta

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.2
-
10 min at 37°C, complete loss of activity, at lower pH values somewhat more stable Macaca mulatta
7 10 10 min, 37°C, stable Macaca mulatta